LOCUS       sequence-100808-        1182 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PhoRadAdC46 intein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1182)
  AUTHORS   Aranko AS, Oeemig JS, Zhou D, Kajander T, Wlodawer A, Iwai H
  TITLE     Structure-based engineering and comparison of novel split inteins 
            for protein ligation.
  JOURNAL   Mol Biosyst. 2014 May;10(5):1023-34. doi: 10.1039/c4mb00021h.
  PUBMED    24574026
REFERENCE   2  (bases 1 to 1182)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Mol Biosyst"; date: "2014-05"; volume: "10"; issue: "5"; pages: 
            "1023-34"
COMMENT     Sequence Label: pSARSF53-344
FEATURES             Location/Qualifiers
     source          1..1182
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     280..299
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        280..298
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    299..323
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             379..396
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             403..693
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="SDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLR
                     RLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIETHREQIGG"
     CDS             1109..1153
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 ccgatgggaa atgtaaatca gctccgccat tcgccgcttt cactttttcc cgcgtgtcgc
       61 aagaaacgtg gctggactgg tcaccacgcg ggaaacggtc tgataaagag acaccgagca
      121 tgctctgcga catcgtataa cgttactggt ttcacaattc accactctga attgactctc
      181 ttccgggcgc tatcatgtca taccgcgaaa aggtttgcgc cattcgatgg tgtccgggat
      241 ctcgacgctc tcccttatgc gactcctgca ttaggaaatt aatacgactc actatagggg
      301 aattgtgagc ggataacaat tcccctgtag aaataatttt gtttaacttt aataaggaga
      361 tataccatgg gcagcagcca tcatcatcat catcacagca ctagtgactc agaagtcaat
      421 caagaagcta agccagaggt caagccagaa gtcaagcctg agactcacat caatttaaag
      481 gtgtccgatg gatcttcaga aatcttcttt aagatcaaaa agaccactcc tttaagaagg
      541 ctgatggaag cgttcgctaa aagacagggt aaggaaatgg actccttaag attcttgtac
      601 gacggtatta gaattcaagc tgatcagacc cctgaagatt tggacatgga ggataacgat
      661 attattgaga ctcacagaga acagattggt ggatccggga agtgctttgc tagggatacc
      721 gaagtttatt atgaaaacga tactgtacca cacatggaat caattgagga gatgtatagt
      781 aaatacgcct cgatgaatgg ggaattacca ttcgataatg gttacgcagt tccattagat
      841 aatgtctttg tgtacacgtt ggacatcgct agtggtgaaa ttaagaaaac gagggcttca
      901 tacatctatc gcgagaaggt tgagaagctc attgagataa aactatccag tggatactcc
      961 cttaaggtta ctccctctca cccagttctc ctctttagag atggcctgca atgggtcccc
     1021 gctgctgaag ttaaacctgg agatgttgtt gttggtgtta gggaagaggt actgagaaga
     1081 taagcttgcg gccgcactcg agtctggtaa agaaaccgct gctgcgaaat ttgaacgcca
     1141 gcacatggac tcgtctacta gcgcagctaa tatcctcttt ta
//
