LOCUS       sequence-10081-e        8496 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8496)
  AUTHORS   Ali S, Curtin JF, Zirger JM, Xiong W, King GD, Barcia C, Liu C, 
            Puntel M, Goverdhana S, Lowenstein PR, Castro MG
  TITLE     Inflammatory and anti-glioma effects of an adenovirus expressing 
            human soluble Fms-like tyrosine kinase 3 ligand (hsFlt3L): treatment
            with hsFlt3L inhibits intracranial glioma progression.
  JOURNAL   Mol Ther. 2004 Dec . 10(6):1071-84.
  PUBMED    15564139
REFERENCE   2  (bases 1 to 8496)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther. 
            2004 Dec . 10(6):1071-84."
COMMENT     Sequence Label: pAL119-Flt3L
FEATURES             Location/Qualifiers
     source          1..8496
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..103
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     promoter        577..780
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     731..751
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     777..801
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    878..934
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     CDS             6048..6239
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(6233..6255)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    6341..6481
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     6396..6415
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6496..6513)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6667..7255)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6747..6766)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7426..8217,8218..8286))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8049..8068
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8287..8391)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8459..8477
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 catcatcaat aatatacctt attttggatt gaagccaata tgataatgag ggggtggagt
       61 ttgtgacgtg gcgcggggcg tgggaacggg gcgggtgacg tagtagtgtg gcggaagtgt
      121 gatgttgcaa gtgtggcgga acacatgtaa gcgacggatg tggcaaaagt gacgtttttg
      181 gtgtgcgccg gtgtanacng gaagtgacaa ttttcgcgcg gttttaggcg gatgttgtag
      241 taaatttggg cgtaaccnag taanatttgg ccattttcgc gggaaaactg aataagagga
      301 agtgaaatct gaataattnt gtgttactca tagcgcgtaa tatttgtcta gggccgcggg
      361 gactttgacc gtttacgtgg agactcgccc aggtgttttt ctcaggtgtt ttccgcgttc
      421 cgggtcaaag ttggcgtttt attattatag tcagctctag actcnagaag cttgcatgcc
      481 tgcaggtcaa ttccctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
      541 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc
      601 aatgggcgtg gatagcggtt tgactcacgg ggatttncaa gtctccaccc cattgacgtc
      661 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc
      721 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct
      781 cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga
      841 agacaccggg accgatccag cctggggatc ttccctgaag cttgcatgcc tgcaggtcga
      901 ctctagagga tccccgggta ccgagctcga attccggggc ccccggccga aatgacagtg
      961 ctggcgccag cctggagccc aacaacctat ctcctcctgc tgctgctgct gagctcggga
     1021 ctcagtggga cccaggactg ctccttccaa cacagcccca tctcctccga cttcgctgtc
     1081 aaaatccgtg agctgtctga ctacctgctt caagattacc cagtcaccgt ggcctccaac
     1141 ctgcaggacg aggagctctg cgggggcctc tggcggctgg tcctggcaca gcgctggatg
     1201 gagcggctca agactgtcgc tgggtccaag atgcaaggct tgctggagcg cgtgaacacg
     1261 gagatacact ttgtcaccaa atgtgccttt cagccccccc ccagctgtct tcgcttcgtc
     1321 cagaccaaca tctcccgcct cctgcaggag acctccgagc agctggtggc gctgaagccc
     1381 tggatcactc gccagaactt ctcccggtgc ctggagctgc agtgtcagcc cgtagagacg
     1441 gtgtttcacc gtgtcagcca ggatggtctc gatctcctga cctcgtgatc tgcccgcctc
     1501 ggcctcccaa agtgctagga ttacagatac tcctcaaccc tgccaccccc atggagtccc
     1561 cggcccctgg aggccacagc cccgacagcc ccgcagcccc ctctgctcct cctactgctg
     1621 ctgcccgtgg gcctcctgct gctggccgct gcctggtgcc tgcactggca gaggacgcgg
     1681 cggaggacac cccgccctgg ggagcaggtg ccccccgtcc ccagtcccca ggacctgctg
     1741 cttgtggagc actgacctgg ccaaggcctc atcctgcgga gccttaaaca acgcagtgag
     1801 acagacatct atcatcccat tttacagggg aggatactga ggcacacaga ggggagtcac
     1861 cagccagagg atgcatagcc tggacacaga ggaagttggc tagaggccgg tcccttcctt
     1921 gggcccctct cattccctcc ccagaatgga ggcaacgcca gaatccagca ccggccccat
     1981 ttacccaact ctgtacaaag cccccggaat tcatcgatga tatcagatcc cacgtcacta
     2041 ttgtatactc tatattatac tctatgttat actctgtaat cctactcaat aaacgtgtca
     2101 cgcctgtgaa accgtactaa gtctcccgtg tcttcttatc accatcaggt gacatcctcg
     2161 cccaggctgt caatcatgcc ggtatcgatt ccagtagcac cggccccacg ctgacaaccc
     2221 actcttgcag cgttagcagc gcccctctta acaagccgac ccccaccagc gtcgcggtta
     2281 ctaacactcc tctccccgac ctgcagccca agcttctcga gtctagagtg gaaggtgctg
     2341 aggtacgatg agacccgcac caggtgcaga ccctgcgagt gtggcggtaa acatattagg
     2401 aaccagcctg tgatgctgga tgtgaccgag gagctgaggc ccgatcactt ggtgctggcc
     2461 tgcacccgcg ctgagtttgg ctctagcgat gaagatacag attgaggtac tgaaatgtgt
     2521 gggcgtggct taagggtggg aaagaatata taaggtgggg gtcttatgta gttttgtatc
     2581 tgttttgcag cagccgccgc cgccatgagc accaactcgt ttgatggaag cattgtgagc
     2641 tcatatttga caacgcgcat gcccccatgg gccggggtgc gtcagaatgt gatgggctcc
     2701 agcattgatg gtcgccccgt cctgcccgca aactctacta ccttgaccta cgagaccgtg
     2761 tctggaacgc cgttggagac tgcagcctcc gccgccgctt cagccgctgc agccaccgcc
     2821 cgcgggattg tgactgactt tgctttcctg agcccgcttg caagcagtgc agcttcccgt
     2881 tcatccgccc gcgatgacaa gttgacggct cttttggcac aattggattc tttgacccgg
     2941 gaacttaatg tcgtttctca gcagctgttg gatctgcgcc agcaggtttc tgccctgaag
     3001 gcttcctccc ctcccaatgc ggtttaaaac ataaataaaa aaccagactc tgtttggatt
     3061 tggatcaagc aagtgtcttg ctgtctttat ttaggggttt tgcgcgcgcg gtaggcccgg
     3121 gaccagcggt ctcggtcgtt gagggtcctg tgtatttttt ccaggacgtg gtaaaggtga
     3181 ctctggatgt tcagatacat gggcataagc ccgtctctgg ggtggaggta gcaccactgc
     3241 agagcttcat gctgcggggt ggtgttgtag atgatccagt cgtagcagga gcgctgggcg
     3301 tggtgcctaa aaatgtcttt cagtagcaag ctgattgcca ggggcaggcc cttggtgtaa
     3361 gtgtttacaa agcggttaag ctgggatggg tgcatacgtg gggatatgag atgcatcttg
     3421 gactgtattt ttaggttggc tatgttccca gccatatccc tccggggatt catgttgtgc
     3481 agaaccacca gcacagtgta tccggtgcac ttgggaaatt tgtcatgtag cttagaagga
     3541 aatgcgtgga agaacttgga gacgcccttg tgacctccaa gattttccat gcattcgtcc
     3601 ataatgatgg caatgggccc acgggcggcg gcctgggcga agatatttct gggatcacta
     3661 acgtcatagt tgtgttccag gatgagatcg tcataggcca tttttacaaa gcgcgggcgg
     3721 agggtgccag actgcggtat aatggttcca tccggcccag gggcgtagtt accctcacag
     3781 atttgcattt cccacgcttt gagttcagat ggggggatca tgtctacctg cggggcgatg
     3841 aagaaaacgg tttccggggt aggggagatc agctgggaag aaagcaggtt cctgagcagc
     3901 tgcgacttac cgcagccggt gggcccgtaa atcacaccta ttaccggctg caactggtag
     3961 ttaagagagc tgcagctgcc gtcatccctg agcagggggg ccacttcgtt aagcatgtcc
     4021 ctgactcgca tgttttccct gaccaaatcc gccagaaggc gctcgccgcc cagcgatagc
     4081 agttcttgca aggaagcaaa gtttttcaac ggtttgagac cgtccgccgt aggcatgctt
     4141 ttgagcgttt gaccaagcag ttccaggcgg tcccacagct cggtcacctg ctctacggca
     4201 tctcgatcca gcatatctcc tcgtttcgcg ggttggggcg gctttcgctg tacggcagta
     4261 gtcggtgctc gtccagacgg gccagggtca tgtctttcca cgggcgcagg gtcctcgtca
     4321 gcgtagtctg ggtcacggtg aaggggtgcg ctccgggctg cgcgctggcc agggtgcgct
     4381 tgaggctggt cctgctggtg ctgaagcgct gccggtcttc gccctgcgcg tcggccaggt
     4441 agcatttgac catggtgtca tagtccagcc cctccgcggc gtggcccttg gcgcgcagct
     4501 tgcccttgga ggaggcgccg cacgaggggc agtgcagact tttgagggcg tagagcttgg
     4561 gcgcgagaaa taccgattcc ggggagtagg catccgcgcc gcaggccccg cagacggtct
     4621 cgcattccac gagccaggtg agctctggcc gttcggggtc aaaaaccagg tttcccccat
     4681 gctttttgat gcgtttctta cctctggttt ccatgagccg gtgtccacgc tcggtgacga
     4741 aaaggctgtc cgtgtccccg tatacagact tgagaggcct gtcctcgacc gatgcccttg
     4801 agagccttca acccagtcag ctccttccgg tgggcgcggg gcatgactat cgtcgccgca
     4861 cttatgactg tcttctttat catgcaactc gtaggacagg tgccggcagc gctctgggtc
     4921 attttcggcg aggaccgctt tcgctggagc gcgacgatga tcggcctgtc gcttgcggta
     4981 ttcggaatct tgcacgccct cgctcaagcc ttcgtcactg gtcccgccac caaacgtttc
     5041 ggcgagaagc aggccattat cgccggcatg gcggccgacg cgctgggcta cgtcttgctg
     5101 gcgttcgcga cgcgaggctg gatggccttc cccattatga ttcttctcgc ttccggcggc
     5161 atcgggatgc ccgcgttgca ggccatgctg tccaggcagg tagatgacga ccatcaggga
     5221 cagcttcaag gatcgctcgc ggctcttacc agcctaactt cgatcactgg accgctgatc
     5281 gtcacggcga tttatgccgc ctcggcgagc acatggaacg ggttggcatg gattgtaggc
     5341 gccgccctat accttgtctg cctccccgcg ttgcgtcgcg gtgcatggag ccgggccacc
     5401 tcgacctgaa tggaagccgg cggcacctcg ctaacggatt caccactcca agaattggag
     5461 ccaatcaatt cttgcggaga actgtgaatg cgcaaaccaa cccttggcag aacatatcca
     5521 tcgcgtccgc catctccagc agccgcacgc ggcgcatctc gggcagcgtt gggtcctggc
     5581 cacgggtgcg catgatcgtg ctcctgtcgt tgaggacccg gctaggctgg cggggttgcc
     5641 ttactggtta gcagaatgaa tcaccgatac gcgagcgaac gtgaagcgac tgctgctgca
     5701 aaacgtctgc gacctgagca acaacatgaa tggtcttcgg tttccgtgtt tcgtaaagtc
     5761 tggaaacgcg gaagtcagcg ccctgcacca ttatgttccg gatctgcatc gcaggatgct
     5821 gctggctacc ctgtggaaca cctacatctg tattaacgaa gcgctggcat tgaccctgag
     5881 tgatttttct ctggtcccgc cgcatccata ccgccagttg tttaccctca caacgttcca
     5941 gtaaccgggc atgttcatca tcagtaaccc gtatcgtgag catcctctct cgtttcatcg
     6001 gtatcattac ccccatgaac agaaatcccc cttacacgga ggcatcagtg accaaacagg
     6061 aaaaaaccgc ccttaacatg gcccgcttta tcagaagcca gacattaacg cttctggaga
     6121 aactcaacga gctggacgcg gatgaacagg cagacatctg tgaatcgctt cacgaccacg
     6181 ctgatgagct ttaccgcagc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac
     6241 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
     6301 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac
     6361 gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag
     6421 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
     6481 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     6541 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     6601 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     6661 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     6721 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     6781 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     6841 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     6901 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     6961 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     7021 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     7081 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
     7141 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     7201 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     7261 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     7321 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     7381 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     7441 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     7501 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     7561 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     7621 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     7681 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     7741 tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     7801 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     7861 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     7921 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     7981 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     8041 aacacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     8101 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     8161 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     8221 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     8281 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     8341 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     8401 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
     8461 taggcgtatc acgaggccct ttcgtcttca agaatt
//
