LOCUS       sequence-100840-         851 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  for CRISPR/Cas9 mediated insertion of E2A-KalTA4; to be used in 
            combination with a eGFP specific sgRNA (e.g. Plasmid 61051).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Auer TO, Duroure K, De Cian A, Concordet JP, Del Bene F
  TITLE     Highly efficient CRISPR/Cas9-mediated knock-in in zebrafish by 
            homology-independent DNA repair.
  JOURNAL   Genome Res. 2014 Jan;24(1):142-53. doi: 10.1101/gr.161638.113. Epub 
            2013 Oct 31.
  PUBMED    24179142
REFERENCE   2  (bases 1 to 851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gr.161638.113"; journalName: "Genome Res"; date: "2014-01";
            volume: "24"; issue: "1"; pages: "142-53"
COMMENT     Sequence Label: eGFPbait-E2A-KalTA4-pA donor vector
FEATURES             Location/Qualifiers
     source          1..851
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    138..272
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(175..194)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     229..248
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 atacgactca ctatagggcg aattgggccc tctagatgca tgctcgagtc cactagttct
       61 agagcggccg cgaattaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
      121 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
      181 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
      241 aactcatcaa tgtatcttat catgtctgga tctacgatcc gtcgaggaat taattctttg
      301 ccaaaatgat gagacagcac aacaaccagc acgttgccca ggagctgtag gaaagagaag
      361 aaggcatgaa catggttagc agaggggccc ggtttggact cagagtattt tatcctcatc
      421 tcaaacagtg tatatcattg taaccataaa gagaaaggca ggatgatgac cagggtgtag
      481 ttgtttctac caataagaat atttccacgc cagccagaat ttatatgcag aaatattcta
      541 ccttatcatt taattataac aattgttctc taaaactgtg ctgaagtaca atataatata
      601 ccctgattgc cttgaaaaaa aagtgattag agaaagtact tacaatctga caaataaaca
      661 aaagtgaatt taaaaattcg ttacaaatgc aagctaaagt ttaacgaaaa agttacagaa
      721 aatgaaaaga aaataagagg agacaatggt tgtcaacaga gtagaaagtg aaagaaacaa
      781 aattatcatg agggtccatg gtgatacaag ggacatcttc ccattctaaa caacaccctg
      841 aaaactttgc c
//
