LOCUS       sequence-100898-         754 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses mCherry-ALK1 in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 754)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 754)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-ALK1-C-13
FEATURES             Location/Qualifiers
     source          1..754
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      103..112
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(151..354)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(180..200)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        355..658
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 cctcgatctc gaactcgtgg ccgttcacgg agccctccat gtgcaccttg aagcgcatga
       61 actccttgat gatggccatg ttatcctcct cgcccttgct caccatggtg gcgaccggta
      121 gcgctagcgg atctgacggt tcactaaacc agctctgctt atatagacct cccaccgtac
      181 acgcctaccg cccatttgcg tcaatggggc ggagttgtta cgacattttg gaaagtcccg
      241 ttgattttgg tgccaaaaca aactcccatt gacgtcaatg gggtggagac ttggaaatcc
      301 ccgtgagtca aaccgctatc cacgcccatt gatgtactgc caaaaccgca tcaccatggt
      361 aatagcgatg actaatacgt agatgtactg ccaagtagga aagtcccata aggtcatgta
      421 ctgggcataa tgccaggcgg gccatttacc gtcattgacg tcaatagggg gcgtacttgg
      481 catatgatac acttgatgta ctgccaagtg ggcagtttac cgtaaatact ccacccattg
      541 acgtcaatgg aaagtcccta ttggcgttac tatgggaaca tacgtcatta ttgacgtcaa
      601 tgggcggggg tcgttgggcg gtcagccagg cgggccattt accgtaagtt atgtaacgcg
      661 gaactccata tatgggctat gaactaatga ccccgtaatt gattactatt aataactaat
      721 gcatggcggt aatacggtta tccacagaat cagg
//
