LOCUS       sequence-100899-         708 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses both fragments of T7 RNAP split at position 179. Both 
            fragments are driven by PBAD. The C-terminal fragment has the point 
            mutation Q758C. Contains a constitutive araC ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Shis DL, Bennett MR
  TITLE     Library of synthetic transcriptional AND gates built with split T7 
            RNA polymerase mutants.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):5028-33. doi: 
            10.1073/pnas.1220157110. Epub 2013 Mar 11.
  PUBMED    23479654
REFERENCE   2  (bases 1 to 708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1220157110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-03-26- 26"; volume: "110"; issue: "13"; pages: "5028-33"
COMMENT     Sequence Label: pTSara-Q758C
FEATURES             Location/Qualifiers
     source          1..708
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(43..70)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 attaataatc tggcttttta tattctctct ctagtatata aacgcagaaa ggcccacccg
       61 aaggtgagcc agtgtgatcc aagccagctc gatatccgtc ggcttgaacg aattgttaga
      121 cattacttgt agacgtgccc tacgcgcttg ttgagttgtt cctcaacgtt tttcttgaag
      181 tgcttagctt caaggtcacg gatacgaccg aagcgagcct cgtcctcaat ggcccgaccg
      241 attgcgcttg ctacagcctg aacggttgta ttgtcagcac tggttaggca agccagagtg
      301 gtcttaatgg tgatgtacgc tacggcttcc ggcttgattt cttgcaggaa ctggaaggct
      361 gtcgggcgct tgccgcgctt agctttcact tcctcaaacc agtcgttgat gcgtgcaatc
      421 atcttaggga gtagggtagt gatgagaggc ttggcggcag cgttatccgc aacctcacca
      481 gctttaagtt gacgctcaaa catcttgcgg aagcgtgctt cacccatctc gtaagactca
      541 tgctcaaggg ccaactgttc gcgagctaaa cgctcaccgt aatggtcagc cagagtgttg
      601 aacgggatag cagccagttc gatgtcagag aagtcgttct tagcgatgtt aatcgtgttc
      661 atattagaaa aagaatgtta ttaagtaagt ctttctagag ctcgctag
//
