LOCUS       sequence-100902-         857 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MethyloBrick expression vector, tuf promoter; TcR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Schada von Borzyskowski L, Remus-Emsermann M, Weishaupt R, Vorholt 
            JA, Erb TJ
  TITLE     A Set of Versatile Brick Vectors and Promoters for the Assembly, 
            Expression, and Integration of Synthetic Operons in Methylobacterium
            extorquens AM1 and Other Alphaproteobacteria.
  JOURNAL   ACS Synth Biol. 2014 Aug 8.
  PUBMED    25105793
REFERENCE   2  (bases 1 to 857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2014 Aug 8."
COMMENT     Sequence Label: pTE105
FEATURES             Location/Qualifiers
     source          1..857
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(437..454)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(437..453)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(446..468)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(662..681)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     781..803
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
       61 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatggaa ttctgtacat
      121 ctagatgcat gctcgagcgg ccgccagtgt gatggatatc tgcagaattc gcccttagtg
      181 aagacaaagg taagcttgct cgatctcggc gagcgccgcg gcggcgcccc ggaggcgcag
      241 cggctctacg agcaggtcgg cgaggccgcg tcctgacccc tcattcggcg cgatccgccc
      301 tgccggcaaa cctgccgctt gatcatcgtc gcacaagcct ctaatcggtc cgcgccttta
      361 cgcccggctt atggccggtc ccgcggtagg attgcacgaa gcttagatct tgactagtcc
      421 tgcaggtacc taattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
      481 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
      541 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga
      601 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca
      661 gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
      721 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
      781 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg
      841 gcctcgtgat acgccta
//
