LOCUS       sequence-100961-         894 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  The codon-optimized Cas9 nuclease under the control of the 
            Drosophila hsp70 promoter used in Gratz, et al. (2013). Plasmid is 
            low copy number..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Gratz SJ, Cummings AM, Nguyen JN, Hamm DC, Donohue LK, Harrison MM, 
            Wildonger J, O'Connor-Giles KM
  TITLE     Genome engineering of Drosophila with the CRISPR RNA-guided Cas9 
            nuclease.
  JOURNAL   Genetics. 2013 May 24.
  PUBMED    23709638
REFERENCE   2  (bases 1 to 894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2013 May 24."
COMMENT     Sequence Label: pHsp70-Cas9
FEATURES             Location/Qualifiers
     source          1..894
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        63..515
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     462..483
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     CDS             534..599
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             606..626
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tttgatcttt tctactgaac ggtgatcccc accggaattg cggccgcgga attcccatcc
       61 ccctagaatc ccaaaacaaa ctggttattg tggtaggtca tttgtttggc agaaagaaaa
      121 ctcgagaaat ttctctggcc gttattcgtt attctctctt ttctttttgg gtctctccct
      181 ctctgcacta atgctctctc actctgtcac acagtaaacg gcatactgct ctcgttggtt
      241 cgagagagcg cgcctcgaat gttcgcgaaa agagcgccgg agtataaata gaggcgcttc
      301 gtctacggag cgacaattca attcaaacaa gcaaagtgaa cacgtcgcta agcgaaagct
      361 aagcaaataa acaagcgcag ctgaacaagc taaacaatct gcagtaaagt gcaagttaaa
      421 gtgaatcaat taaaagtaac cagcaaccaa gtaaatcaac tgcaactact gaaatctgcc
      481 aagaagtaat tattgaatac aagaagagaa ctctggggga tctgatcgat atggactata
      541 aggaccacga cggagactac aaggatcatg atattgatta caaagacgat gacgataaga
      601 tggccccaaa gaagaagcgg aaggtcggta tccacggagt cccagcagcc gacaagaagt
      661 acagcatcgg cctggacatc ggcaccaact ctgtgggctg ggccgtgatc accgacgagt
      721 acaaggtgcc cagcaagaaa ttcaaggtgc tgggcaacac cgaccggcac agcatcaaga
      781 agaacctgat cggagccctg ctgttcgaca gcggcgaaac agccgaggcc acccggctga
      841 agagaaccgc cagaagaaga tacaccagac ggaagaaccg gatctgctat ctgc
//
