LOCUS       sequence-100975-         768 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Goulas T, Cuppari A, Garcia-Castellanos R, Snipas S, Glockshuber R, 
            Arolas JL, Gomis-Ruth FX
  TITLE     The pCri System: A Vector Collection for Recombinant Protein 
            Expression and Purification.
  JOURNAL   PLoS One. 2014 Nov 11;9(11):e112643. doi: 
            10.1371/journal.pone.0112643. eCollection 2014.
  PUBMED    25386923
REFERENCE   2  (bases 1 to 768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-11-11"; volume: "9(11):e112643. doi"; pages: " 
            10.1371/journal.pone.0112643. eCollection 2014"
COMMENT     Sequence Label: pCri-16a
FEATURES             Location/Qualifiers
     source          1..768
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             68..85
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      165..411
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
ORIGIN
        1 gagctgtaca agtaaggtac cggatccgaa ttcgagctcc gtcgacaagc ttgcggccgc
       61 actcgagcat catcatcatc atcattgagt ttgtagcctt agacatgact gttcctcagt
      121 tcaagttggg cacttacgag aagaccggtc ttgctagatt ctaatcaaga ggatgtcaga
      181 atgccatttg cctgagagat gcaggcttca tttttgatac ttttttattt gtaacctata
      241 tagtatagga ttttttttgt cattttgttt cttctcgtac gagcttgctc ctgatcagcc
      301 tatctcgcag ctgatgaata tcttgtggta ggggtttggg aaaatcattc gagtttgatg
      361 tttttcttgg tatttcccac tcctcttcag agtacagaag attaagtgag accttcgttt
      421 gtgcggatcc cccacacacc atagcttcaa aatgtttcta ctcctttttt actcttccag
      481 attttctcgg actccgcgca tcgccgtacc acttcaaaac acccaagcac agcatactaa
      541 atttcccctc tttcttcctc tagggtgtcg ttaattaccc gtactaaagg tttggaaaag
      601 aaaaaagaga ccgcctcgtt tctttttctt cgtcgaaaaa ggcaataaaa atttttatca
      661 cgtttctttt tcttgaaaat tttttttttt gatttttttc tctttcgatg acctcccatt
      721 gatatttaag ttaataaacg gtcttcaatt tctcaagttt cagtttca
//
