LOCUS       sequence-100998-        1083 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a tandem GFP-APEX2 fusion to the C-terminus of connexin43 
            in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Lam SS, Martell JD, Kamer KJ, Deerinck TJ, Ellisman MH, Mootha VK, 
            Ting AY
  TITLE     Directed evolution of APEX2 for electron microscopy and proximity 
            labeling.
  JOURNAL   Nat Methods. 2014 Nov 24. doi: 10.1038/nmeth.3179.
  PUBMED    25419960
REFERENCE   2  (bases 1 to 1083)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Nov 24. doi: 10.1038/nmeth.3179."
COMMENT     Sequence Label: pcDNA3 Connexin43-GFP-APEX2
FEATURES             Location/Qualifiers
     source          1..1083
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      115..124
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc tggctagcgt ttaaacttaa gcttgccacc
      121 atgggtgact ggagtgcctt ggggaaatta ctggacaagg tccaagccta ctccaccgct
      181 ggagggaagg tgtggctgtc agtgctcttc atattcagaa tcctgctcct ggggacagct
      241 gttgagtcag cttggggtga tgaacagtct gcctttcgct gtaacactca acaacctggc
      301 tgcgaaaacg tctgctatga caagtccttc cccatctctc acgtgcgctt ctgggtcctt
      361 cagatcatat tcgtgtctgt gcccacactc ctgtacttgg cccatgtgtt ctatgtgatg
      421 aggaaggaag agaagctaaa caagaaagaa gaggagctca aagtggccca gactgacggg
      481 gtcaacgtgg agatgcacct gaagcagatt gaaatcaaga agttcaagta cgggattgaa
      541 gagcacggca aggtgaaaat gaggggcggc ttgctgagaa cctacatcat cagcatcctc
      601 ttcaagtctg tcttcgaggt ggccttcctg ctcatccagt ggtacatcta tgggttcagc
      661 ttgagcgcgg tctacacctg caagagagat ccctgccccc accaggtaga ctgcttcctc
      721 tcacgtccca cggagaaaac catcttcatc atcttcatgc tggtggtgtc cttggtgtct
      781 ctcgctttga acatcattga gctcttctac gtcttcttca agggcgttaa ggatcgcgtg
      841 aagggaagaa gcgatcctta ccacgccacc actggcccac tgagcccatc aaaagactgc
      901 ggatctccaa aatacgccta cttcaatggc tgctcctcac caacggctcc actctcgcct
      961 atgtctcctc ctgggtacaa gctggttact ggtgacagaa acaattcctc gtgccgcaat
     1021 tacaacaagc aagctagcga gcaaaactgg gcgaactaca gcgcagagca aaatcgcatg
     1081 ggg
//
