LOCUS       sequence-101005-         856 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Golgi TGN, Excitation: 399, Emission: 456.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Subach OM, Cranfill PJ, Davidson MW, Verkhusha VV
  TITLE     An enhanced monomeric blue fluorescent protein with the high 
            chemical stability of the chromophore.
  JOURNAL   PLoS One. 2011;6(12):e28674. Epub 2011 Dec 8.
  PUBMED    22174863
REFERENCE   2  (bases 1 to 856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(12)"; pages: "e28674. Epub 2011 Dec 8"
COMMENT     Sequence Label: mTagBFP2-SiT-N-15
FEATURES             Location/Qualifiers
     source          1..856
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      10..19
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(253..456)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(282..302)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        457..760
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 ccttagacac catggtggcg accggtggat cccgggcccg cggtaccgtc gactgcagga
       61 attccttggt ttgcaattta aaggaatcat agtaactccc tttcttcttt tccttccaca
      121 cacagatgac tgcaaacaga agaaagacca ggacgcagca gctgaacttt ttcttcaggt
      181 tggtgtgaat catggtggcg agctcgagat ctgagtccgg tagcgctagc ggatctgacg
      241 gttcactaaa ccagctctgc ttatatagac ctcccaccgt acacgcctac cgcccatttg
      301 cgtcaatggg gcggagttgt tacgacattt tggaaagtcc cgttgatttt ggtgccaaaa
      361 caaactccca ttgacgtcaa tggggtggag acttggaaat ccccgtgagt caaaccgcta
      421 tccacgccca ttgatgtact gccaaaaccg catcaccatg gtaatagcga tgactaatac
      481 gtagatgtac tgccaagtag gaaagtccca taaggtcatg tactgggcat aatgccaggc
      541 gggccattta ccgtcattga cgtcaatagg gggcgtactt ggcatatgat acacttgatg
      601 tactgccaag tgggcagttt accgtaaata ctccacccat tgacgtcaat ggaaagtccc
      661 tattggcgtt actatgggaa catacgtcat tattgacgtc aatgggcggg ggtcgttggg
      721 cggtcagcca ggcgggccat ttaccgtaag ttatgtaacg cggaactcca tatatgggct
      781 atgaactaat gaccccgtaa ttgattacta ttaataacta atgcatggcg gtaatacggt
      841 tatccacaga atcagg
//
