LOCUS       sequence-101006-         786 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Goulas T, Cuppari A, Garcia-Castellanos R, Snipas S, Glockshuber R, 
            Arolas JL, Gomis-Ruth FX
  TITLE     The pCri System: A Vector Collection for Recombinant Protein 
            Expression and Purification.
  JOURNAL   PLoS One. 2014 Nov 11;9(11):e112643. doi: 
            10.1371/journal.pone.0112643. eCollection 2014.
  PUBMED    25386923
REFERENCE   2  (bases 1 to 786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-11-11"; volume: "9(11):e112643. doi"; pages: " 
            10.1371/journal.pone.0112643. eCollection 2014"
COMMENT     Sequence Label: pCri-9a
FEATURES             Location/Qualifiers
     source          1..786
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             68..85
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(138..156)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      152..199
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      236..691
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(323..342)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     533..554
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gagctgtaca agtaaggtac cggatccgaa ttcgagctcc gtcgacaagc ttgcggccgc
       61 actcgagcac caccaccacc accactgaga tccggctgct aacaaagccc gaaaggaagc
      121 tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg
      181 ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggattggcga atgggacgcg
      241 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
      301 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
      361 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
      421 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
      481 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
      541 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
      601 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
      661 aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg
      721 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgaattaat
      781 tcttag
//
