LOCUS       sequence-10101-e        2031 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of photoactivatable Rac1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Wu YI, Frey D, Lungu OI, Jaehrig A, Schlichting I, Kuhlman B, Hahn 
            KM
  TITLE     A genetically encoded photoactivatable Rac controls the motility of 
            living cells.
  JOURNAL   Nature. 2009 Sep 3. 461(7260):104-8.
  PUBMED    19693014
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2009 Sep 3. 461(7260):104-8."
COMMENT     Sequence Label: pTriEx-mVenus-PA-Rac1
FEATURES             Location/Qualifiers
     source          1..2031
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        7..105
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     CDS             127..144
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             join(151..153,154..864)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(193..214)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(454..473)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     801..822
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             877..1305
                     /codon_start=1
                     /gene="NPH1 from Avena sativa"
                     /product="light, oxygen, or voltage domain from phototropin
                     1 of Avena sativa (Herrou and Crosson, 2011)"
                     /label=LOV2
                     /note="photoswitch that undergoes a light-inducible 
                     conformational change"
                     /translation="LATTLERIEKNFVITDPRLPDNPIIFASDSFLQLTEYSREEILGR
                     NCRFLQGPETDRATVRKIRDAIDNQTEVTVQLINYTKSGKKFWNLFHLQPMRDQKGDVQ
                     YFIGVQLDGTEHVRDAAEREGVMLIKKTAENIDEAAKEL"
     CDS             1912..1944
                     /codon_start=1
                     /product="HSV (herpes simplex virus) epitope tag"
                     /label=HSV tag
                     /translation="QPELAPEDPED"
     CDS             1951..1974
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 tttaattcaa cccaacacaa tatattatag ttaaataaga attattatca aatcatttgt
       61 atattaatta aaatactata ctgtaaatta cattttattt acaatcaaag gagatatacc
      121 atggcacacc atcaccacca tcacggatct gtgagcaagg gcgaggagct gttcaccggg
      181 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
      241 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttgat ctgcaccacc
      301 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tcggctacgg cctgcagtgc
      361 ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
      421 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
      481 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
      541 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
      601 tatatcaccg ccgacaagca gaagaacggc atcaaggcaa acttcaagat ccgccacaac
      661 atcgaggacg gcggcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
      721 ggccccgtgc tgctgcccga caaccactac ctgagctacc agtccaaact gagcaaagac
      781 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
      841 ctcggcatgg acgagctgta caagggttct ggatccttgg ctactacact tgaacgtatt
      901 gagaagaact ttgtcattac tgacccaagg ttgccagata atcccattat attcgcgtcc
      961 gatagtttct tgcagttgac agaatatagc cgtgaagaaa ttttgggaag aaactgcagg
     1021 tttctacaag gtcctgaaac tgatcgcgcg acagtgagaa aaattagaga tgccatagat
     1081 aaccaaacag aggtcactgt tcagctgatt aattatacaa agagtggtaa aaagttctgg
     1141 aacctctttc acttgcagcc tatgcgagat cagaagggag atgtccagta ctttattggg
     1201 gttcagttgg atggaactga gcatgtccga gatgctgccg agagagaggg agtcatgctg
     1261 attaagaaaa ctgcagaaaa tattgatgag gcggcaaaag aacttatcaa gtgtgtggtg
     1321 gtgggagacg gagctgtagg taaaacttgc ctactgatca gttacacaac caatgcattt
     1381 cctggagaat atatccctac tgtctttgac aattattctg ccaatgttat ggtagatgga
     1441 aaaccggtga atctgggctt atgggataca gctggactag aagattatga cagattacgc
     1501 cccctatcct atccgcaaac agatgtgttc ttaatttgct tttcccttgt gagtcctgca
     1561 tcattccacc acgtccgtgc aaagtggtat cctgaggtgc ggcaccactg tcccaacact
     1621 cccatcatcc tagtgggaac taaacttgat cttagggatg ataaagacac gatcgagaaa
     1681 ctgaaggaga agaagctgac tcccatcacc tatccgcagg gtctagccat ggctaaggag
     1741 attggtgctg taaaatacct ggagtgctcg gcgctcacac agcgaggcct caagacagtg
     1801 tttgacgaag cgatccgagc agtcctctgc ccgcctcccg tgaagaagag gaagagaaaa
     1861 tgcctgctgt tgtaaaagct tgcggccgca cagctgtata cacgtgcaag ccagccagaa
     1921 ctcgccccgg aagaccccga ggatctcgag caccaccatc accatcacca tcactaagtg
     1981 attaacctca ggtgcaggct gcctatcaga aggtggtggc tggtgtggcc a
//
