LOCUS       sequence-101020-         693 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Goulas T, Cuppari A, Garcia-Castellanos R, Snipas S, Glockshuber R, 
            Arolas JL, Gomis-Ruth FX
  TITLE     The pCri System: A Vector Collection for Recombinant Protein 
            Expression and Purification.
  JOURNAL   PLoS One. 2014 Nov 11;9(11):e112643. doi: 
            10.1371/journal.pone.0112643. eCollection 2014.
  PUBMED    25386923
REFERENCE   2  (bases 1 to 693)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-11-11"; volume: "9(11):e112643. doi"; pages: " 
            10.1371/journal.pone.0112643. eCollection 2014"
COMMENT     Sequence Label: pCri-15b
FEATURES             Location/Qualifiers
     source          1..693
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             82..111
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             127..144
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      223..469
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
ORIGIN
        1 gtaaggtacc ggatccgaat tcgagctccg tcgacaagct tgcggccgca ctcgagccgc
       61 ggcggccgcc agcttgggcc cgaacaaaaa ctcatctcag aagaggatct gaatagcgcc
      121 gtcgaccatc atcatcatca tcattgagtt ttagccttag acatgactgt tcctcagttc
      181 aagttgggca cttacgagaa gaccggtctt gctagattct aatcaagagg atgtcagaat
      241 gccatttgcc tgagagatgc aggcttcatt tttgatactt ttttatttgt aacctatata
      301 gtataggatt ttttttgtca ttttgtttct tctcgtacga gcttgctcct gatcagccta
      361 tctcgcagct gatgaatatc ttgtggtagg ggtttgggaa aatcattcga gtttgatgtt
      421 tttcttggta tttcccactc ctcttcagag tacagaagat taagtgagac cttcgtttgt
      481 gcggatcccc cacacaccat agcttcaaaa tgtttctact ccttttttac tcttccagat
      541 tttctcggac tccgcgcatc gccgtaccac ttcaaaacac ccaagcacag catactaaat
      601 ttcccctctt tcttcctcta gggtgtcgtt aattacccgt actaaaggtt tggaaaagaa
      661 aaaagagacc gcctcgtttc tttttcttcg tcg
//
