LOCUS       sequence-101111-         959 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of tdTomato controlled by the ubiquitously 
            active CMV promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Waldner C, Roose M, Ryffel GU
  TITLE     Red fluorescent Xenopus laevis: a new tool for grafting analysis.
  JOURNAL   BMC Dev Biol. 2009 Jun 23;9:37. doi: 10.1186/1471-213X-9-37.
  PUBMED    19549299
REFERENCE   2  (bases 1 to 959)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Dev 
            Biol."; date: "2009-06-23"; volume: "9:37. doi"; pages: " 
            10.1186/1471-213X-9-37"
COMMENT     Sequence Label: pCSCMV:tdTomato
FEATURES             Location/Qualifiers
     source          1..959
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        63..81
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     63..80
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      114..123
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(253..271)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     521..540
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     715..738
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 ctatataagc aatgctcgtt tagggaaccg ccattctgcc tggggacgtc ggagcaagct
       61 tgatttaggt gacactatag aatacaagct acttgttctt tttgcaggat cccgccacca
      121 tggtgagcaa gggcgaggag gtcatcaaag agttcatgcg cttcaaggtg cgcatggagg
      181 gctccatgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg
      241 gcacccagac cgccaagctg aaggtgacca agggcggccc cctgcccttc gcctgggaca
      301 tcctgtcccc ccagttcatg tacggctcca aggcgtacgt gaagcacccc gccgacatcc
      361 ccgattacaa gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg
      421 aggacggcgg tctggtgacc gtgacccagg actcctccct gcaggacggc acgctgatct
      481 acaaggtgaa gatgcgcggc accaacttcc cccccgacgg ccccgtaatg cagaagaaga
      541 ccatgggctg ggaggcctcc accgagcgcc tgtacccccg cgacggcgtg ctgaagggcg
      601 agatccacca ggccctgaag ctgaaggacg gcggccacta cctggtggag ttcaagacca
      661 tctacatggc caagaagccc gtgcaactgc ccggctacta ctacgtggac accaagctgg
      721 acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgagcgc tccgagggcc
      781 gccaccacct gttcctgggg catggcaccg gcagcaccgg cagcggcagc tccggcaccg
      841 cctcctccga ggacaacaac atggccgtca tcaaagagtt catgcgcttc aaggtgcgca
      901 tggagggctc catgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccc
//
