LOCUS       sequence-101112-        1027 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of tdTomato controlled by the ubiquitously 
            active CMV promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Waldner C, Roose M, Ryffel GU
  TITLE     Red fluorescent Xenopus laevis: a new tool for grafting analysis.
  JOURNAL   BMC Dev Biol. 2009 Jun 23;9:37. doi: 10.1186/1471-213X-9-37.
  PUBMED    19549299
REFERENCE   2  (bases 1 to 1027)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Dev 
            Biol."; date: "2009-06-23"; volume: "9:37. doi"; pages: " 
            10.1186/1471-213X-9-37"
COMMENT     Sequence Label: pCSCMV:tdTomato
FEATURES             Location/Qualifiers
     source          1..1027
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     216..233
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    350..371
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(386..391,392..409,410..416)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    424..440
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     429..451
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     448..464
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     448..464
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     483..503
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        485..503
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     polyA_signal    616..750
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(653..672)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     707..726
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     783..799
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     784..800
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(907..930)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
       61 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      121 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      181 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
      241 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
      301 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
      361 agctcactca ttaggcaccc caggctttac actttatgct cccggctcgt atgttgtgtg
      421 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
      481 gcgcaattaa ccctcactaa agggaacaaa agctgggtac cgggcccaat gcattggcgc
      541 cgcggccgcg aattaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
      601 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
      661 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
      721 ctcatcaatg tatcttatca tgtctggatc tacgtaatac gactcactat agttctagag
      781 gctcgaggtc gacggtatcg ataagcttga tatcgaattc ttacttgtac agctcgtcca
      841 tgccgtacag gaacaggtgg tggcggccct cggagcgctc gtactgttcc acgatggtgt
      901 agtcctcgtt gtgggaggtg atgtccagct tggtgtccac gtagtagtag ccgggcagtt
      961 gcacgggctt cttggccatg tagatggtct tgaactccac caggtagcgg ccgccgtcct
     1021 tcagctt
//
