LOCUS       sequence-101164-        1033 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Probe for HSPB7 for in situ hybridization in zebrafish.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Marvin M, O'Rourke D, Kurihara T, Juliano CE, Harrison KL, Hutson LD
  TITLE     Developmental expression patterns of the zebrafish small heat shock 
            proteins.
  JOURNAL   Dev Dyn. 2008 Feb;237(2):454-63.
  PUBMED    18161059
REFERENCE   2  (bases 1 to 1033)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Dyn."; 
            date: "2008-02"; volume: "237(2)"; pages: "454-63"
COMMENT     Sequence Label: pCRII HSPB7
FEATURES             Location/Qualifiers
     source          1..1033
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        22..40
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     22..39
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(708..727)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(709..727)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(734..751)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(734..750)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(743..765)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(978..997)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tgaccatgat tacgccaagc tatttaggtg acactataga atactcaagc tatgcatcaa
       61 gcttggtacc gagctcggat ccactagtaa cggccgccag tgtgctggaa ttcgccctta
      121 gatgtggacc ccacttcagt gaagtccagt ttaggggccg atgggactct gactataaaa
      181 gcacagagaa acacagctaa actggagcat gcacagacgt tccgcacaga gatcaaaatc
      241 tagacctgtg ccctcctgct gtgtctgttt cactgtgata aatgaccttg aaaatatagc
      301 tacttagccc tgctttgtca ttcctccatg accttctctc atttctgctc attagacttg
      361 gatggaattt aatcctgtca catgtattca agacctttaa gatgaatgca ctgctaacgt
      421 cagctctctg ctaatgacat atcagcttac aggacaaagt gggctccaaa attctggaag
      481 tacatttaat ccaaaatata attgaaacat tgaaagatat cagaatatat atggctatac
      541 agtagatata gtactttagt actttaaatt acatttagtt cacccaaaca tgtaaatcct
      601 gccattattt actcatttcc acttgttcca aacccaataa gggcgaattc tgcagatatc
      661 catcacactg gcggccgctc gagcatgcat ctagagggcc caattcgccc tatagtgagt
      721 cgtattacaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
      781 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
      841 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg acgcgccctg
      901 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
      961 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     1021 ctttccccgt caa
//
