LOCUS       sequence-101191-        8232 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human immunoglobulin epsilon antibody expression vector (kappa light
            chain) without variable regions. Enables Colony-PCR screening of 
            false-positive (PCR vector template) colonies.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8232)
  AUTHORS   Dodev TS, Karagiannis P, Gilbert AE, Josephs DH, Bowen H, James LK, 
            Bax HJ, Beavil R, Pang MO, Gould HJ, Karagiannis SN, Beavil AJ
  TITLE     A tool kit for rapid cloning and expression of recombinant 
            antibodies.
  JOURNAL   Sci Rep. 2014 Jul 30;4:5885. doi: 10.1038/srep05885.
  PUBMED    25073855
REFERENCE   2  (bases 1 to 8232)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2014-07-30"; volume: "4:5885. doi"; pages: " 
            10.1038/srep05885"
COMMENT     Sequence Label: pVITRO1-dV-IgE/kappa
FEATURES             Location/Qualifiers
     source          1..8232
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        51..242
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(74..94)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        256..1562
                     /label=mEF-1-alpha promoter
                     /note="strong constitutive promoter for mouse elongation 
                     factor EF-1-alpha"
     intron          583..1530
                     /label=mEF-1-alpha intron
                     /note="intron upstream of the start codon of mouse 
                     EF-1-alpha"
     polyA_signal    2955..3076
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2966..2985)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3020..3039
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      3264..3852
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3753..3772
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        3932..4235
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4350..5655
                     /label=rEF-1-alpha promoter
                     /note="strong constitutive promoter for rat elongation 
                     factor EF-1-alpha"
     misc_feature    4692..5623
                     /label=rEF-1-alpha intron
                     /note="intron upstream of the start codon of rat 
                     EF-1-alpha"
     misc_feature    6079..6523
                     /label=FMDV IRES
                     /note="internal ribosome entry site (IRES) of the 
                     foot-and-mouth disease virus"
     promoter        6556..6602
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        6594..6612
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             6622..7647
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    7659..8231
                     /label=EF-1-alpha poly(A) signal
                     /note="human EF-1-alpha polyadenylation signal"
ORIGIN
        1 cctgcagggc ctgaaataac ctctgaaaga ggaacttggt taggtacctt ctgaggcgga
       61 aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
      121 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
      181 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
      241 ccactagtgg agccgagagt aattcataca aaaggaggga tcgccttcgc aaggggagag
      301 cccagggacc gtccctaaat tctcacagac ccaaatccct gtagccgccc cacgacagcg
      361 cgaggagcat gcgctcaggg ctgagcgcgg ggagagcaga gcacacaagc tcatagaccc
      421 tggtcgtggg ggggaggacc ggggagctgg cgcggggcaa actgggaaag cggtgtcgtg
      481 tgctggctcc gccctcttcc cgagggtggg ggagaacggt atataagtgc ggcagtcgcc
      541 ttggacgttc tttttcgcaa cgggtttgcc gtcagaacgc aggtgagggg cgggtgtggc
      601 ttccgcgggc cgccgagctg gaggtcctgc tccgagcggg ccgggccccg ctgtcgtcgg
      661 cggggattag ctgcgagcat tcccgcttcg agttgcgggc ggcgcgggag gcagagtgcg
      721 aggcctagcg gcaaccccgt agcctcgcct cgtgtccggc ttgaggccta gcgtggtgtc
      781 cgcgccgccg ccgcgtgcta ctccggccgc actctggtct tttttttttt tgttgttgtt
      841 gccctgctgc cttcgattgc cgttcagcaa taggggctaa caaagggagg gtgcggggct
      901 tgctcgcccg gagcccggag aggtcatggt tggggaggaa tggagggaca ggagtggcgg
      961 ctggggcccg cccgccttcg gagcacatgt ccgacgccac ctggatgggg cgaggcctgg
     1021 ggtttttccc gaagcaacca ggctggggtt agcgtgccga ggccatgtgg ccccagcacc
     1081 cggcacgatc tggcttggcg gcgccgcgtt gccctgcctc cctaactagg gtgaggccat
     1141 cccgtccggc accagttgcg tgcgtggaaa gatggccgct cccgggccct gttgcaagga
     1201 gctcaaaatg gaggacgcgg cagcccggtg gagcgggcgg gtgagtcacc cacacaaagg
     1261 aagagggcct ggtccctcac cggctgctgc ttcctgtgac cccgtggtcc tatcggccgc
     1321 aatagtcacc tcgggctttt gagcacggct agtcgcggcg gggggagggg atgtaatggc
     1381 gttggagttt gttcacattt ggtgggtgga gactagtcag gccagcctgg cgctggaagt
     1441 catttttgga atttgtcccc ttgagttttg agcggagcta attctcgggc ttcttagcgg
     1501 ttcaaaggta tcttttaaac ccttttttag gtgttgtgaa aaccaccgct aattcaaagc
     1561 aaccggtatg gactggacct ggaggatcct cttcttggtg gcggccgcca caggcgcgca
     1621 ctccgaattc gatatcctcg aggctagcac acagagccca tccgtcttcc ccttgacccg
     1681 ctgctgcaaa aacattccct ccaatgccac ctccgtgact ctgggctgcc tggccacggg
     1741 ctacttcccg gagccggtga tggtgacctg ggacacaggc tccctcaacg ggacaactat
     1801 gaccttacca gccaccaccc tcacgctctc tggtcactat gccaccatca gcttgctgac
     1861 cgtctcgggt gcgtgggcca agcagatgtt cacctgccgt gtggcacaca ctccatcgtc
     1921 cacagactgg gtcgacaaca aaaccttcag cgtctgctcc agggacttca ccccgcccac
     1981 cgtgaagatc ttacagtcgt cctgcgacgg cggcgggcac ttccccccga ccatccagct
     2041 cctgtgcctc gtctctgggt acaccccagg gactatcaac atcacctggc tggaggacgg
     2101 gcaggtcatg gacgtggact tgtccaccgc ctctaccacg caggagggtg agctggcctc
     2161 cacacaaagc gagctcaccc tcagccagaa gcactggctg tcagaccgca cctacacctg
     2221 ccaggtcacc tatcaaggtc acacctttga ggacagcacc aagaagtgtg cagattccaa
     2281 cccgagaggg gtgagcgcct acctaagccg gcccagcccg ttcgacctgt tcatccgcaa
     2341 gtcgcccacg atcacctgtc tggtggtgga cctggcaccc agcaagggga ccgtgaacct
     2401 gacctggtcc cgggccagtg ggaagcctgt gaaccactcc accagaaagg aggagaagca
     2461 gcgcaatggc acgttaaccg tcacgtccac cctgccggtg ggcacccgag actggatcga
     2521 gggggagacc taccagtgca gggtgaccca cccccacctg cccagggccc tcatgcggtc
     2581 cacgaccaag accagcggcc cgcgtgctgc cccggaagtc tatgcgtttg cgacgccgga
     2641 gtggccgggg agccgggaca agcgcaccct cgcctgcctg atccagaact tcatgcctga
     2701 ggacatctcg gtgcagtggc tgcacaacga ggtgcagctc ccggacgccc ggcacagcac
     2761 gacgcagccc cgcaagacca agggctccgg cttcttcgtc ttcagccgcc tggaggtgac
     2821 cagggccgaa tgggagcaga aagatgagtt catctgccgt gcagtccatg aggcagcgag
     2881 cccctcacag accgtccagc gagcggtgtc tgtaaatccc ggtaaatgat gtacagctag
     2941 ctggccagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg
     3001 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag
     3061 ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga
     3121 ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg aaatgttaat
     3181 taactagcca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     3241 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     3301 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     3361 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     3421 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     3481 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     3541 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     3601 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     3661 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     3721 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     3781 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     3841 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     3901 tttgctcaca tgttcttaat taacctgcag gcgttacata acttacggta aatggcccgc
     3961 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
     4021 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
     4081 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg
     4141 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc
     4201 agtacatcta cgtattagtc atcgctatta ccatgatgat gcggttttgg cagtacatca
     4261 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca
     4321 atgggagttt gttttgacta gtggagccga gagtaattca tacaaaagga gggatcgcct
     4381 tcgcaagggg agagcccagg gaccgtccct aaattctcac agacccaaat ccctgtagcc
     4441 gccccacgac agcgcgagga gcatgcgccc agggctgagc gcgggtagat cagagcacac
     4501 aagctcacag tccccggcgg tggggggagg ggcgcgctga gcgggggcca gggagctggc
     4561 gcggggcaaa ctgggaaagt ggtgtcgtgt gctggctccg ccctcttccc gagggtgggg
     4621 gagaacggta tataagtgcg gtagtcgcct tggacgttct ttttcgcaac gggtttgccg
     4681 tcagaacgca ggtgagtggc gggtgtggct tccgcgggcc ccggagctgg agccctgctc
     4741 tgagcgggcc gggctgatat gcgagtgtcg tccgcagggt ttagctgtga gcattcccac
     4801 ttcgagtggc gggcggtgcg ggggtgagag tgcgaggcct agcggcaacc ccgtagcctc
     4861 gcctcgtgtc cggcttgagg cctagcgtgg tgtccgccgc cgcgtgccac tccggccgca
     4921 ctatgcgttt tttgtccttg ctgccctcga ttgccttcca gcagcatggg ctaacaaagg
     4981 gagggtgtgg ggctcactct taaggagccc atgaagctta cgttggatag gaatggaagg
     5041 gcaggagggg cgactggggc ccgcccgcct tcggagcaca tgtccgacgc cacctggatg
     5101 gggcgaggcc tgtggctttc cgaagcaatc gggcgtgagt ttagcctacc tgggccatgt
     5161 ggccctagca ctgggcacgg tctggcctgg cggtgccgcg ttcccttgcc tcccaacaag
     5221 ggtgaggccg tcccgcccgg caccagttgc ttgcgcggaa agatggccgc tcccggggcc
     5281 ctgttgcaag gagctcaaaa tggaggacgc ggcagcccgg tggagcgggc gggtgagtca
     5341 cccacacaaa ggaagagggc cttgcccctc gccggccgct gcttcctgtg accccgtggt
     5401 ctatcggccg catagtcacc tcgggcttct cttgagcacc gctcgtcgcg gcggggggag
     5461 gggatctaat ggcgttggag tttgttcaca tttggtgggt ggagactagt caggccagcc
     5521 tggcgctgga agtcattctt ggaatttgcc cctttgagtt tggagcgagg ctaattctca
     5581 agcctcttag cggttcaaag gtattttcta aacccgtttc caggtgttgt gaaagccacc
     5641 gctaattcaa agcaatccgg aatgttgcca tcacaactca ttgggtttct gctgctctgg
     5701 gttccagcta gccgcggtga attcgatatc ctcgagcgta cggtggcggc gccatctgtc
     5761 ttcatcttcc cgccatctga tgagcagttg aaatctggaa ctgcctctgt tgtgtgcctg
     5821 ctgaataact tctatcccag agaggccaaa gtacagtgga aggtggataa cgccctccaa
     5881 tcgggtaact cccaggagag tgtcacagag caggacagca aggacagcac ctacagcctc
     5941 agcagcaccc tgacgctgag caaagcagac tacgagaaac acaaagtcta cgcctgcgaa
     6001 gtcacccatc agggcctgag ctcgcccgtc acaaagagct tcaacagggg agagtgttag
     6061 ggatcccgta cgcctaggag caggtttccc caatgacaca aaacgtgcaa cttgaaactc
     6121 cgcctggtct ttccaggtct agaggggtaa cactttgtac tgcgtttggc tccacgctcg
     6181 atccactggc gagtgttagt aacagcactg ttgcttcgta gcggagcatg acggccgtgg
     6241 gaactcctcc ttggtaacaa ggacccacgg ggccaaaagc cacgcccaca cgggcccgtc
     6301 atgtgtgcaa ccccagcacg gcgactttac tgcgaaaccc actttaaagt gacattgaaa
     6361 ctggtaccca cacactggtg acaggctaag gatgcccttc aggtaccccg aggtaacacg
     6421 cgacactcgg gatctgagaa ggggactggg gcttctataa aagcgctcgg tttaaaaagc
     6481 ttctatgcct gaataggtga ccggaggtcg gcacctttcc tttgcaatta ctgaccctat
     6541 gaatacactg actgtttgac aattaatcat cggcatagta tatcggcata gtataatacg
     6601 actcactata ggagggccac catgaagaaa cctgaactga cagcaacttc tgttgagaag
     6661 tttctcattg aaaaatttga ttctgtttct gatctcatgc agctgtctga aggtgaagaa
     6721 agcagagcct tttcttttga tgttggagga agaggttatg ttctgagggt caattcttgt
     6781 gctgatggtt tttacaaaga cagatatgtt tacagacact ttgcctctgc tgctctgcca
     6841 attccagaag ttctggacat tggagaattt tctgaatctc tcacctactg catcagcaga
     6901 agagcacaag gagtcactct ccaggatctc cctgaaactg agctgccagc tgttctgcaa
     6961 cctgttgctg aagcaatgga tgccattgca gcagctgatc tgagccaaac ctctggattt
     7021 ggtccttttg gtccccaagg cattggtcag tacaccactt ggagggattt catttgtgcc
     7081 attgctgatc ctcatgtcta tcactggcag actgtgatgg atgacacagt ttctgcttct
     7141 gttgctcagg cactggatga actcatgctg tgggcagaag attgtcctga agtcagacac
     7201 ctggtccatg ctgattttgg aagcaacaat gttctgacag acaatggcag aatcactgca
     7261 gtcattgact ggtctgaagc catgtttgga gattctcaat atgaggttgc caacattttt
     7321 ttttggagac cttggctggc ttgcatggaa caacaaacaa gatattttga aagaagacac
     7381 ccagaactgg ctggttcccc cagactgaga gcctacatgc tcagaattgg cctggaccaa
     7441 ctgtatcaat ctctggttga tggaaacttt gatgatgctg cttgggcaca aggaagatgt
     7501 gatgccattg tgaggtctgg tgctggaact gttggaagaa ctcaaattgc aagaaggtct
     7561 gctgctgttt ggactgatgg atgtgttgaa gttctggctg actctggaaa caggagaccc
     7621 tccacaagac ccagagccaa ggaatgaata ttagctagat tatccctaat acctgccacc
     7681 ccactcttaa tcagtggtgg aagaacggtc tcagaactgt ttgtttcaat tggccattta
     7741 agtttagtag taaaagactg gttaatgata acaatgcatc gtaaaacctt cagaaggaaa
     7801 ggagaatgtt ttgtggacca ctttggtttt cttttttgcg tgtggcagtt ttaagttatt
     7861 agtttttaaa atcagtactt tttaatggaa acaacttgac caaaaatttg tcacagaatt
     7921 ttgagaccca ttaaaaaagt taaatgagaa acctgtgtgt tcctttggtc aacaccgaga
     7981 catttaggtg aaagacatct aattctggtt ttacgaatct ggaaacttct tgaaaatgta
     8041 attcttgagt taacacttct gggtggagaa tagggttgtt ttccccccac ataattggaa
     8101 ggggaaggaa tatcatttaa agctatggga gggttgcttt gattacaaca ctggagagaa
     8161 atgcagcatg ttgctgattg cctgtcacta aaacaggcca aaaactgagt ccttgggttg
     8221 catagaaagc tg
//
