LOCUS       sequence-101227-         886 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 plasmid with sgRNA deleted; for use in C. elegans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Ward JD
  TITLE     Rapid and Precise Engineering of the Caenorhabditis elegans Genome 
            with Lethal Mutation Co-conversion and Inactivation of NHEJ Repair.
  JOURNAL   Genetics. 2014 Dec 9. pii: genetics.114.172361.
  PUBMED    25491644
REFERENCE   2  (bases 1 to 886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2014 Dec 9. pii: genetics.114.172361."
COMMENT     Sequence Label: pJW1259
FEATURES             Location/Qualifiers
     source          1..886
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(334..360)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(361..381)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ttttcttggc ggcacaataa agtttcctct ttttgttggg tcactcaacg aacacaatac
       61 aaatatttga gcgctcgcat ttttctcaac ctttcgggtt taaagcactt caaaatggaa
      121 ttgaagagag attgtgaaag tacatcagac agaatcacat tttgatagca ttcacttcac
      181 tcagatgcaa acttttcagt gactataaaa agtcataaaa taataacatt aaaaaagcaa
      241 ataaattagc gagagaattt tttgacaaaa agaaagaaga gtgatagaga agaagggaat
      301 gcttgaaagg attttgcatt tatcactagt ttaggcgtag tctgggacgt cgtatgggta
      361 gaccttacgc ttcttctttg gtggtccgga tcctccgtct cctccgagtt gggagaggtc
      421 gatacgggtc tcgtagagtc cggtgatgga ttggtggatg agggtggcgt cgaggacctc
      481 cttggtggag gtgtaacgct tacggtcgat ggtggtgtcg aagtacttga aggcggctgg
      541 ggctccgagg ttggtgaggg tgaagaggtg gatgatgttc tcggcttgct cacggattgg
      601 cttgtcacgg tgcttgttgt aggcggagag gaccttgtcg aggttggcgt cggcgaggat
      661 gacacgcttg gagaactcgg agatttgctc gatgatctcg tcgaggtagt gcttgtgttg
      721 ctcgacgaag agttgctttt gctcgttgtc ctctggggat cccttgagct tctcgtagtg
      781 ggaggcgagg tagaggaagt tgacgtactt ggatgggagg gcgagctcgt ttcccttttg
      841 gagctctccg gcggaggcga gcatacgctt acgtccgttc tcgagc
//
