LOCUS       sequence-101316-         823 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Template for cloning U6 promoter to generate new PU6::sgRNAs by 
            PCR-fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Ward JD
  TITLE     Rapid and Precise Engineering of the Caenorhabditis elegans Genome 
            with Lethal Mutation Co-conversion and Inactivation of NHEJ Repair.
  JOURNAL   Genetics. 2014 Dec 9. pii: genetics.114.172361.
  PUBMED    25491644
REFERENCE   2  (bases 1 to 823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2014 Dec 9. pii: genetics.114.172361."
COMMENT     Sequence Label: pJW1310
FEATURES             Location/Qualifiers
     source          1..823
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     237..253
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     260..279
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        260..278
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(350..699)
                     /label=CeU6 promoter
                     /note="promoter for the C. elegans R07E5.16 U6 snRNA gene 
                     (Dickinson et al., 2013)"
     primer_bind     720..736
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    744..764
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 gatcaccatc cgtcgccccg gcgtgtcaat aatatcactc tgtacatcca caaacagacg
       61 ataacggctc tctcttttat aggtgtaaac cttaaactgc cgtacgtata ggctgcgcaa
      121 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg
      181 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa
      241 aacgacggcc agtgaattgt aatacgactc actatagggc gaattgggcc ctctagatgc
      301 atgctcgagc ggccgccagt gtgatggata tctgcagaat tcgcccttca agacatctcg
      361 caataggagg tgtttatata ggggcaacac aagaggcggc agacatctcg tgagtttgac
      421 agagtagtat aaagagtgtg tgtgagaggg tgtaggcatt tcataacata ttttttgtgg
      481 aataattttg gaaaaaaggt gtttttttaa taatagcttt tataagagtt tctcaaaaat
      541 tgcatgcttt ttttgaaaag gttgacaaat tatagtagta ctaaagttgc tattttcagg
      601 tctcagcaga aaattcacaa ctaaaaataa aatttgcaaa acagagtgtg cgcctttaag
      661 gtgtacagtt ttttattcct ttattgctag ttttttttgg ttttcccagt cacgacgttg
      721 taaaacgacg gccagtgaat tatcaactat gtataataaa gttgtgagac ttttttcttg
      781 gcggcacaat aaagtttcct ctttttgttg ggtcactcaa cga
//
