LOCUS       sequence-101320-         745 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses S. pyogenes Cas9 plus an HDV ribozyme-sgRNA for genome 
            editing in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Ryan OW, Skerker JM, Maurer MJ, Li X, Tsai JC, Poddar S, Lee ME, 
            DeLoache W, Dueber JE, Arkin AP, Cate JH
  TITLE     Selection of chromosomal DNA libraries using a multiplex CRISPR 
            system.
  JOURNAL   Elife. 2014 Aug 19;3. doi: 10.7554/eLife.03703.
  PUBMED    25139909
REFERENCE   2  (bases 1 to 745)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2014-08-19"; volume: "3. doi"; pages: " 10.7554/eLife.03703"
COMMENT     Sequence Label: pCAS
FEATURES             Location/Qualifiers
     source          1..745
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        156..231
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        365..708
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
ORIGIN
        1 cgcaagactg taatttatca ctacgaaatc ttgagatcgg gcgttcgact cgcccccggg
       61 agagatggcc ggcatggtcc cagcctcctc gctggcgccg gctgggcaac accttcgggt
      121 ggcgaatggg actttcataa taacgtccaa taaatgtttt agagctagaa atagcaagtt
      181 aaaataaggc tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg ctttttttat
      241 tttttgtcac tattgttatg taaaatgcca cctctgacag tatggaacgc aaacttctgt
      301 ctagtggata taggtctaga gatctgttta gcttgcctcg tccccgccgg gtcacccggc
      361 cagcgacatg gaggcccaga ataccctcct tgacagtctt gacgtgcgca gctcaggggc
      421 atgatgtgac tgtcgcccgt acatttagcc catacatccc catgtataat catttgcatc
      481 catacatttt gatggccgca cggcgcgaag caaaaattac ggctcctcgc tgcagacctg
      541 cgagcaggga aacgctcccc tcacagacgc gttgaattgt ccccacgccg cgcccctgta
      601 gagaaatata aaaggttagg atttgccact gaggttcttc tttcatatac ttccttttaa
      661 aatcttgcta ggatacagtt ctcacatcac atccgaacat aaacaaccat gggtaaggaa
      721 aagactcacg tttcgaggcc gcgat
//
