LOCUS       sequence-101321-         671 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses S. pyogenes Cas9 plus an HDV ribozyme-sgRNA for genome 
            editing in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Ryan OW, Skerker JM, Maurer MJ, Li X, Tsai JC, Poddar S, Lee ME, 
            DeLoache W, Dueber JE, Arkin AP, Cate JH
  TITLE     Selection of chromosomal DNA libraries using a multiplex CRISPR 
            system.
  JOURNAL   Elife. 2014 Aug 19;3. doi: 10.7554/eLife.03703.
  PUBMED    25139909
REFERENCE   2  (bases 1 to 671)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2014-08-19"; volume: "3. doi"; pages: " 10.7554/eLife.03703"
COMMENT     Sequence Label: pCAS
FEATURES             Location/Qualifiers
     source          1..671
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(251..298)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
ORIGIN
        1 gtctaaagag taatactaga gataaacata aaaaatgtag aggtcgagtt tagatgcaag
       61 ttcaaggagc gaaaggtgga tgggtaggtt atatagggat atagcacaga gatatatagc
      121 aaagagatac ttttgagcaa tgtttgtgga agcggtattc gcaatatttt agtagcccgt
      181 tacagtccgg tgcgtttttg gttttttgaa agtgcgtctt cagagcgctt ttggttttca
      241 aaagcgctct gaagttccta tactttctag agaataggaa cttcggaata ggaacttcaa
      301 agcgtttccg aaaacgagcg cttccgaaaa tgcaacgcga gctgcgcaca tacagctcac
      361 tgttcacgtc gcacctatat ctgcgtgttg cctgtatata tatatacatg agaagaacgg
      421 catagtgcgt gtttatgctt aaatgcgtat atgtgttatg tagtatactc tttcttcaac
      481 aattaaatac tctcggtagc caagttggtt taaggcgcaa gactgtaatt tatcactacg
      541 aaatcttgag atcgggcgtt cgactcgccc ccgggagaga tggccggcat ggtcccagcc
      601 tcctcgctgg cgccggctgg gcaacacctt cgggtggcga atgggacttt cataataacg
      661 tccaataaat g
//
