LOCUS       sequence-101342-         679 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Template for cloning U6 promoter to generate new PU6::sgRNAs by 
            PCR-fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Ward JD
  TITLE     Rapid and Precise Engineering of the Caenorhabditis elegans Genome 
            with Lethal Mutation Co-conversion and Inactivation of NHEJ Repair.
  JOURNAL   Genetics. 2014 Dec 9. pii: genetics.114.172361.
  PUBMED    25491644
REFERENCE   2  (bases 1 to 679)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2014 Dec 9. pii: genetics.114.172361."
COMMENT     Sequence Label: pJW1310
FEATURES             Location/Qualifiers
     source          1..679
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     192..209
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    326..347
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(362..367,368..385,386..392)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    400..416
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     405..427
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     424..440
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     424..440
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        458..476
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     458..475
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    complement(622..642)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(650..667)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(650..666)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
       61 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
      121 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
      181 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
      241 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
      301 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
      361 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
      421 acacaggaaa cagctatgac catgattacg ccaagctatt taggtgacac tatagaatac
      481 tcaagctatg catcaagctt ggtaccgagc tcggatccac tagtaacggc cgccagtgtg
      541 ctggaattcg cccttattgt gttcgttgag tgacccaaca aaaagaggaa actttattgt
      601 gccgccaaga aaaaagtctc acaactttat tatacatagt tgataattca ctggccgtcg
      661 ttttacaacg tcgtgactg
//
