LOCUS       sequence-101348-         734 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Goulas T, Cuppari A, Garcia-Castellanos R, Snipas S, Glockshuber R, 
            Arolas JL, Gomis-Ruth FX
  TITLE     The pCri System: A Vector Collection for Recombinant Protein 
            Expression and Purification.
  JOURNAL   PLoS One. 2014 Nov 11;9(11):e112643. doi: 
            10.1371/journal.pone.0112643. eCollection 2014.
  PUBMED    25386923
REFERENCE   2  (bases 1 to 734)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-11-11"; volume: "9(11):e112643. doi"; pages: " 
            10.1371/journal.pone.0112643. eCollection 2014"
COMMENT     Sequence Label: pCri-15b
FEATURES             Location/Qualifiers
     source          1..734
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             675..692
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             702..722
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 tggcgaggtt catgtttgtt tatttccgaa tgcaacaagc tccgcattac acccgaacat
       61 cactccagat gagggctttc tgagtgtggg gtcaaatagt ttcatgttcc ccaaatggcc
      121 caaaactgac agtttaaacg ctgtcttgga acctaatatg acaaaagcgt gatctcatcc
      181 aagatgaact aagtttggtt cgttgaaatg ctaacggcca gttggtcaaa aagaaacttc
      241 caaaagtcgg cataccgttt gtcttgtttg gtattgattg acgaatgctc aaaaataatc
      301 tcattaatgc ttagcgcagt ctctctatcg cttctgaacc ccggtgcacc tgtgccgaaa
      361 cgcaaatggg gaaacacccg ctttttggat gattatgcat tgtctccaca ttgtatgctt
      421 ccaagattct ggtgggaata ctgctgatag cctaacgttc atgatcaaaa tttaactgtt
      481 ctaaccccta cttgacagca atatataaac agaaggaagc tgccctgtct taaacctttt
      541 tttttatcat cattattagc ttactttcat aattgcgact ggttccaatt gacaagcttt
      601 tgattttaac gacttttaac gacaacttga gaagatcaaa aaacaactaa ttattcgaaa
      661 cgatgggcag cagccatcat catcatcatc acagcagcgg cgaaaacctt tacttccagg
      721 gccatatggc tagc
//
