LOCUS       sequence-101387-        3098 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MuLE (Multiple Lentiviral Expression) Entry vector containing a SFFV
            promoter and multiple cloning site module. Compatible with MultiSite
            Gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, 
            Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, 
            Bode-Lesniewska B, Wild PJ, Frew IJ
  TITLE     A versatile modular vector system for rapid combinatorial mammalian 
            genetics.
  JOURNAL   J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743.
  PUBMED    25751063
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743."
COMMENT     Sequence Label: pMuLE ENTR SFFV L5-L2
FEATURES             Location/Qualifiers
     source          1..3098
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(85..184)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(214..233)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(215..233)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(238..254)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(238..254)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(356..375)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             367..1176
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(434..453)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      1323..1911
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1812..1831
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2065..2082
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2241..2268)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2360..2446)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2495..2517
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2509..2526
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2510..2526
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2543..2638
                     /gene="mutant version of attL"
                     /label=attL5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 gatccactag taacggccgc cagtgtgctg gaattctgca gatatccatc acactggcgg
       61 ccgctcgagc atgcatctag aggtacccag ctttcttgta caaagttggc attataagaa
      121 agcattgctt atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta
      181 tttgccatcc agctgcaggg cggccgcgat atcccctata gtgagtcgta ttacatggtc
      241 atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa
      301 gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag taatacaagg
      361 ggtgttatga gccatattca acgggaaacg tcgaggccgc gattaaattc caacatggat
      421 gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc
      481 tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc
      541 gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct
      601 cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg
      661 atccccggaa aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt
      721 gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct
      781 tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg
      841 gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa
      901 gaaatgcata aacttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca
      961 cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc
     1021 ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct
     1081 ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa
     1141 ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa ttggttgtaa
     1201 cactggcaga gcattacgct gacttgacgg gacggcgcaa gctcatgacc aaaatccctt
     1261 aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     1321 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     1381 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     1441 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     1501 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     1561 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     1621 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     1681 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     1741 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     1801 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     1861 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     1921 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     1981 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     2041 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     2101 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     2161 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atacgcgtac cgctagccag
     2221 gaagagtttg tagaaacgca aaaaggccat ccgtcaggat ggccttctgc ttagtttgat
     2281 gcctggcagt ttatggcggg cgtcctgccc gccaccctcc gggccgttgc ttcacaacgt
     2341 tcaaatccgc tcccggcgga tttgtcctac tcaggagagc gttcaccgac aaacaacaga
     2401 taaaacgaaa ggcccagtct tccgactgag cctttcgttt tatttgatgc ctggcagttc
     2461 cctactctcg cgttaacgct agcatggatg ttttcccagt cacgacgttg taaaacgacg
     2521 gccagtctta agctcgggcc ccaaataatg attttatttt gactgatagt gacctgttcg
     2581 ttgcaacaaa ttgatgagca atgctttttt ataatgccaa ctttgtatac aaaagttggg
     2641 agacccaagc ttcgctgaaa gaccccacct gtaggtttgg caagctagct gcagtaacgc
     2701 cattttgcaa ggcatggaaa aataccaaac caagaataga gaagttcaga tcaagggcgg
     2761 gtacatgaaa atagctaacg ttgggccaaa caggatatct gcggtgagca gtttcggccc
     2821 cggcccgggg ccaagaacag atggtcaccg cagtttcggc cccggcccga ggccaagaac
     2881 agatggtccc cagatatggc ccaaccctca gcagtttctt aagacccatc agatgtttcc
     2941 aggctccccc aaggacctga aatgaccctg cgccttattt gaattaacca atcagcctgc
     3001 ttctcgcttc tgttcgcgcg cttctgcttc ccgagctcta taaaagagct cacaacccct
     3061 cactcggcgc gccagtcctc cgattgactg agtcgccg
//
