LOCUS       sequence-101492-         887 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 433, Emission: 475.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Markwardt ML, Kremers GJ, Kraft CA, Ray K, Cranfill PJ, Wilson KA, 
            Day RN, Wachter RM, Davidson MW, Rizzo MA
  TITLE     An improved cerulean fluorescent protein with enhanced brightness 
            and reduced reversible photoswitching.
  JOURNAL   PLoS One. 2011  . 6(3):e17896.
  PUBMED    21479270
REFERENCE   2  (bases 1 to 887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2011  . 6(3):e17896."
COMMENT     Sequence Label: mCerulean3-ER-5
FEATURES             Location/Qualifiers
     source          1..887
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    184..305
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(221..240)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     275..294
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(312..767)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(449..470)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     661..680
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 agtccggaaa ggacgagctg taagaattct gcagtcgacg gtaccgcggg cccgggatcc
       61 accggatcta gataactgat cataatcagc cataccacat ttgtagaggt tttacttgct
      121 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt
      181 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
      241 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
      301 tcttaacgcg taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
      361 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
      421 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
      481 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
      541 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
      601 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
      661 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
      721 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt
      781 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
      841 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaa
//
