LOCUS       sequence-101512-         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Orange fluorescent protein based pH indicator in bacterial 
            expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Shen Y, Rosendale M, Campbell RE, Perrais D
  TITLE     pHuji, a pH-sensitive red fluorescent protein for imaging of exo- 
            and endocytosis.
  JOURNAL   J Cell Biol. 2014 Nov 10;207(3):419-32. doi: 10.1083/jcb.201404107.
  PUBMED    25385186
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2014-11-10- 10"; volume: "207"; 
            issue: "3"; pages: "419-32"
COMMENT     Sequence Label: pBAD-pHoran4
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(6..716)
                     /codon_start=1
                     /product="orange monomeric derivative of DsRed fluorescent 
                     protein (Shanet et al., 2004)"
                     /label=mOrange
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPQFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKKRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVGIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(284..303)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             complement(join(732..746,747..755))
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             complement(759..791)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             complement(795..812)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 agcttttact tgtacagctc gtccatgccg ccggtggagt ggcggccctc ggcgcgttcg
       61 tactgttcca cgatggtgta gtcctcgttg tgggaggtga tgtccaactt gatgccgacg
      121 atgtaggcgc cgggcagctg cacgggcttc ttggccttgt aggtggtctt gacctcggag
      181 gtgtagtggc cgccgtcctt cagcttcagc ctcttcttga tctcgccctt cagggcgccg
      241 tcctcggggt acatccgctc ggaggaggcc tcccagccca tggtcttctt ctgcattacg
      301 gggccgtcgg aggggaagtt ggtgccgcgc agcttcacct tgtagatgaa ctcgccgtcc
      361 tgcagggagg agtcctgggt cacggtcacc acgccgccgt cctcgaagtt catcacgcgc
      421 tcccacttga agccctcggg gaaggacagc ttgaagtagt cggggatgtc ggcggggtgc
      481 ttcacgtagg ccttggagcc gtaggtgaac tgaggggaca ggatgtccca ggcgaagggc
      541 agggggccac ccttggtcac cttcagctta gcggtctgaa agccctcgta ggggcggccc
      601 tcgccctcgc cctcgatctc gaactcgtgg ccgttcacgg agccctccat gcgcaccttg
      661 aagcgcatga actccttgat gatggccatg ttattctcct cgcccttgct caccattctc
      721 gagctcggat ccttatcgtc atcgtcgtac agatcccgac ccatttgctg tccaccagtc
      781 atgctagcca taccatgatg atgatgatga tgagaacccc ccatggttaa ttcctcctgt
      841 tagcccaaaa aacgggtatg gagaaac
//
