LOCUS       sequence-101535-         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PK1F GFP reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Segall-Shapiro TH, Meyer AJ, Ellington AD, Sontag ED, Voigt CA
  TITLE     A 'resource allocator' for transcription based on a highly 
            fragmented T7 RNA polymerase.
  JOURNAL   Mol Syst Biol. 2014 Jul 30;10(7):742.
  PUBMED    25080493
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2014-07-30"; volume: "10(7)"; pages: "742"
COMMENT     Sequence Label: pTHSSd_10
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     386..404
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(513..541)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 aactctcaag gatcttaccg ctgttgagat ccagttcgat ataacccact cgtgcaccca
       61 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
      121 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
      181 tttttcaata ttattgaagc atttatcgcg aaacgatcct catcctgtct cttgatcaga
      241 tcttgatccc ctgcgccatc agatccttgg cggcaagaaa gccatccagt ttactttgca
      301 gggcttccca accttaccag agggcgcccc agctggcaat tccgacgtct aagaaaccat
      361 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcacct
      421 cgagtaataa ctatcactat agggagagga tcctgtcaat ttccgcgata gaggaggtaa
      481 agatgagcaa aggagaagaa cttttcactg gagttgtccc aattcttgtt gaattagatg
      541 gtgatgttaa tgggcacaaa ttttctgtcc gtggagaggg tgaaggtgat gctacaaacg
      601 gaaaactcac ccttaaattt atttgcacta ctggaaaact acctgttccg tggccaacac
      661 ttgtcactac tctgacctat ggtgttcaat gcttttcccg ttatccggat cacatgaaac
      721 ggcatgactt tttcaagagt gccatgcccg aaggttatgt acaggaacgc actatatctt
      781 tcaaagatga cgggacctac aagacgcgtg ctgaagtcaa gtttgaaggt gatacccttg
      841 ttaatcgtat cgagttaaag ggtattgatt
//
