LOCUS       sequence-101537-         842 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PK1F GFP reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Segall-Shapiro TH, Meyer AJ, Ellington AD, Sontag ED, Voigt CA
  TITLE     A 'resource allocator' for transcription based on a highly 
            fragmented T7 RNA polymerase.
  JOURNAL   Mol Syst Biol. 2014 Jul 30;10(7):742.
  PUBMED    25080493
REFERENCE   2  (bases 1 to 842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2014-07-30"; volume: "10(7)"; pages: "742"
COMMENT     Sequence Label: pTHSSd_10
FEATURES             Location/Qualifiers
     source          1..842
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(51..764,765..767))
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             complement(54..767)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(94..114)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     709..737
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 ccgtttagag gccccaaggg gttatgctag gatccttatt aagcacctta ttatttgtag
       61 agctcatcca tgccatgtgt aatcccagca gcagttacaa actcaagaag gaccatgtgg
      121 tcacgctttt cgttgggatc tttcgaaagg acagattgtg tcgacaggta atggttgtct
      181 ggtaaaagga cagggccatc gccaattgga gtattttgtt gataatggtc tgctagttga
      241 acggaaccat cttcaacgtt gtggcgaatt ttgaagttag ctttgattcc attcttttgt
      301 ttgtctgccg tgatgtatac attgtgtgag ttaaagttgt actcgagttt gtgtccaaga
      361 atgtttccat cttctttaaa atcaataccc tttaactcga tacgattaac aagggtatca
      421 ccttcaaact tgacttcagc acgcgtcttg taggtcccgt catctttgaa agatatagtg
      481 cgttcctgta cataaccttc gggcatggca ctcttgaaaa agtcatgccg tttcatgtga
      541 tccggataac gggaaaagca ttgaacacca taggtcagag tagtgacaag tgttggccac
      601 ggaacaggta gttttccagt agtgcaaata aatttaaggg tgagttttcc gtttgtagca
      661 tcaccttcac cctctccacg gacagaaaat ttgtgcccat taacatcacc atctaattca
      721 acaagaattg ggacaactcc agtgaaaagt tcttctcctt tgctcatctt tacctcctct
      781 atcgcggaaa ttgacaggat cctctcccta tagtgatagt tattactcga ggtgaagacg
      841 aa
//
