LOCUS       sequence-101538-         847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PK1F GFP reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Segall-Shapiro TH, Meyer AJ, Ellington AD, Sontag ED, Voigt CA
  TITLE     A 'resource allocator' for transcription based on a highly 
            fragmented T7 RNA polymerase.
  JOURNAL   Mol Syst Biol. 2014 Jul 30;10(7):742.
  PUBMED    25080493
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2014-07-30"; volume: "10(7)"; pages: "742"
COMMENT     Sequence Label: pTHSSd_10
FEATURES             Location/Qualifiers
     source          1..847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     199..217
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(326..354)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 atattattga agcatttatc gcgaaacgat cctcatcctg tctcttgatc agatcttgat
       61 cccctgcgcc atcagatcct tggcggcaag aaagccatcc agtttacttt gcagggcttc
      121 ccaaccttac cagagggcgc cccagctggc aattccgacg tctaagaaac cattattatc
      181 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtcttca cctcgagtaa
      241 taactatcac tatagggaga ggatcctgtc aatttccgcg atagaggagg taaagatgag
      301 caaaggagaa gaacttttca ctggagttgt cccaattctt gttgaattag atggtgatgt
      361 taatgggcac aaattttctg tccgtggaga gggtgaaggt gatgctacaa acggaaaact
      421 cacccttaaa tttatttgca ctactggaaa actacctgtt ccgtggccaa cacttgtcac
      481 tactctgacc tatggtgttc aatgcttttc ccgttatccg gatcacatga aacggcatga
      541 ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa cgcactatat ctttcaaaga
      601 tgacgggacc tacaagacgc gtgctgaagt caagtttgaa ggtgataccc ttgttaatcg
      661 tatcgagtta aagggtattg attttaaaga agatggaaac attcttggac acaaactcga
      721 gtacaacttt aactcacaca atgtatacat cacggcagac aaacaaaaga atggaatcaa
      781 agctaacttc aaaattcgcc acaacgttga agatggttcc gttcaactag cagaccatta
      841 tcaacaa
//
