LOCUS       sequence-101556-        1171 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  to overexpress yeast polymerase eta in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   Pavlov YI, Nguyen D, Kunkel TA
  TITLE     Mutator effects of overproducing DNA polymerase eta (Rad30) and its 
            catalytically inactive variant in yeast.
  JOURNAL   Mutat Res. 2001 Jul 1;478(1-2):129-39.
  PUBMED    11406177
REFERENCE   2  (bases 1 to 1171)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mutat 
            Res."; date: "2001-07-1"; volume: "478(1-2)"; pages: "129-39"
COMMENT     Sequence Label: pESC-LEU2-RAD30cmyc
FEATURES             Location/Qualifiers
     source          1..1171
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(50..239)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     CDS             complement(269..298)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggatc ttcgagcgtc
       61 ccaaaacctt ctcaagcaag gttttcagta taatgttaca tgcgtacacg cgtctgtaca
      121 gaaaaaaaag aaaaatttga aatataaata acgttcttaa tactaacata actataaaaa
      181 aataaatagg gacctagact tcaggttgtc taactccttc cttttcggtt agagcggatc
      241 ttagctagcc gcggtaccaa gcttactcga ggtcttcttc ggaaatcaac ttctgttcca
      301 tgtcgacttt ttttcttgta aaaaatgata agatgttttt ggaagatgta acttgtttct
      361 tctgaggtgt ggcagtatgt tgtgagtttg gtcttttccg tgaaaacaac aatctttttt
      421 ccccgaaaga aaggtttttg gatgattctt cagcaccatt taagccttcc gacagtttca
      481 atgctaaatg atagtctgcg tgctcttgaa gagccttctg gtctgtaaaa gtcacctggt
      541 atttacaaca ttccaacttc ggagttttct cgtcagcttt cgagctggta gttttttcct
      601 catcttcctt gcctgctgag cttttgaaag tatggacctg attgccaaac atatctacaa
      661 cagttttttg taaatctata atatcgaaat tagtaattgt catacttagt tttgttaaag
      721 ggtaataact tttgttcttt cctttaatat caaggtccgt gacaaatttt atgcctactt
      781 ttaggagttc gtgactctgg aaattgatgc ccttgtacgc aactggacct gattttcgat
      841 atacttcgta ggattttgtt tttaaagata tagatactgt tcttggtatt acgatcttgt
      901 tatactcctg ttccagatcc tgaatcctgg acgtaagctc agcgcagaag acctcaagcc
      961 aagaaataca gtccacgatg gaattgcacg attttcctct aagatttttg tttgacatca
     1021 tactttttac aacagggcga gaacttaagg gcagaccgta ccggcctcta cttaatttaa
     1081 acaacttttc tgctaaatct gctgttttca aagggtcgat attgctggtt gagcgatcat
     1141 aatcagattg cttaacttta gcatctaaaa a
//
