LOCUS       sequence-101611-         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GeneGuard genomic cassette: for constructing Delta-dapA::RBS E pi, 
            Kis, FRT-kanR-FRT (medium-copy support for R6K COR). Will support 
            pSEVAD1K..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Wright O, Delmans M, Stan GB, Ellis T
  TITLE     GeneGuard: a Modular Plasmid System Designed for Biosafety.
  JOURNAL   ACS Synth Biol. 2014 May 13.
  PUBMED    24847673
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2014 May 13."
COMMENT     Sequence Label: pSEVA12DKR-E
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(380..413)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     RBS             693..701
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     RBS             741..749
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 ttgttcaatc atgcgaaacg atcctcatcc tgtctcttga tcagatcttg atcccctgcg
       61 ccatcagatc cttggcggca agaaagccat ccagtttact ttgcagggct tcccaacctt
      121 accagagggc gccccagctg gcaattccgg ttcgcttgct gtccataaaa ccgcccagtc
      181 tagctatcgc catgtaagcc cactgcaagc tacctgcttt ctctttgcgc ttgcgttttc
      241 ccttgtccag atagcccagt agctgacatt catccggggt cagcaccgtt tctgcggact
      301 ggctttctac gtgttccgct tcctttagca gcccttgcgc cctgagtgct tgcggcagcg
      361 tgagcttcaa aagcgctctg aagttcctat actttctaga gaataggaac ttcgaactgc
      421 aggtcgactc agatttcctc ctgaccagtc gccggtgcat ccagccattc tcgttcttca
      481 gctgatattt cagcattcgg atcacactgt gccagtagct cagccagtga atattgcggg
      541 cgtctgtacg gctcaacaat cagccggcca ttatcaatga ccatgccaac ttcattatct
      601 gtgcccagag acagcgcatt cagcagtgcc ggtgggacgg tcagcataac tgagccgcca
      661 accctcttca gtcgggtggt atgcattaga aaacctcctt agcatgatta agatgtttct
      721 tcaacactcg catggttgtt acctccttac ctctggtctc cacacattat acctataggt
      781 tagactttaa gtcaatactc tttttcccgg gtataaacgc agaaaggccc acccgaa
//
