LOCUS       sequence-101699-         907 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a tandem green ddFP heterodimer in mammalian cells, 
            construct 1 for a green to red colour switch-based fluorescent 
            caspase-3 biosensor, used together with a second plasmid RANLS..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Ding Y, Li J, Enterina JR, Shen Y, Zhang I, Tewson PH, Mo GC, Zhang 
            J, Quinn AM, Hughes TE, Maysinger D, Alford SC, Zhang Y, Campbell RE
  TITLE     Ratiometric biosensors based on dimerization-dependent fluorescent 
            protein exchange.
  JOURNAL   Nat Methods. 2015 Jan 26. doi: 10.1038/nmeth.3261.
  PUBMED    25622108
REFERENCE   2  (bases 1 to 907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Jan 26. doi: 10.1038/nmeth.3261."
COMMENT     Sequence Label: GANES-DEVD-BNLS
FEATURES             Location/Qualifiers
     source          1..907
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(22..42)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(46..66)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(159..182)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(357..376)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     626..644
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 ctttctcttc ttttttggat ctacctttct cttctttttt ggatctacct ttctcttctt
       61 ttttggatcg cttcccgccg ttcccttgta ccgctcgtcc atgcccagac ggtggcggcc
      121 ctcggagcgc ccgtactgtt ccacgatggt gtagtcctcg ttgtgggagg tgatgtccag
      181 cttggtgtcc acacagtaat cgccgggcag ttgcacgggc ttcttggcca tgtagatggt
      241 ctcgaactcc accaggtagt ggccgccgtc cttcagcttc agggcctgat agctatggcc
      301 cttcagcacg ccgtccttgg ggtacagcat ctcggtggag gcctcccagc ccatggtctt
      361 cttctgcatt acggggccgt cgggggggaa gttggtgccg cgaatcttca ccttgcagat
      421 cagcgtgccg tcctgcaggg aggagtcctg ggtaacggtc accagaccgc cgtcttcgaa
      481 gttcatcacg cgctcccact tgaagccctc ggggaagggc agcttcttgt aatcggggat
      541 gtcggcgggg tgcctcacgt acgctttgga gccgtacatg atctgggggg acaggatgtc
      601 ccaggcaaag ggcagggggc cgcccttggt caccttcagc ttggcggtct gggtgccctc
      661 gtaggggcgg ccctcgccct cgccctcgat ctcgaactcg tggccgttca tggagccctc
      721 catgcgcacc ttgaagcgca tgaactcttt gatggtggct ccatccacct catcgccgga
      781 ggcggtaccg ctcccgatat caagacctgc taatttcaag gctaaggtgc tgccggtgcc
      841 atgccccagg tggtggcggc cctcggagcg ctcgtactgt tccacgatgg tgtagtcctc
      901 gttgtgg
//
