LOCUS       sequence-101714-         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FPX biosensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Ding Y, Li J, Enterina JR, Shen Y, Zhang I, Tewson PH, Mo GC, Zhang 
            J, Quinn AM, Hughes TE, Maysinger D, Alford SC, Zhang Y, Campbell RE
  TITLE     Ratiometric biosensors based on dimerization-dependent fluorescent 
            protein exchange.
  JOURNAL   Nat Methods. 2015 Jan 26. doi: 10.1038/nmeth.3261.
  PUBMED    25622108
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Jan 26. doi: 10.1038/nmeth.3261."
COMMENT     Sequence Label: single polypeptide FPX biosensor for calcium ion
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(82..105)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(280..299)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     549..567
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 cgctgcctgt gctacctgta ccatgcccca ggtggtggcg gccctcggag cgctcgtact
       61 gttccacgat ggtgtagtcc tcgttgtggg aggtgatgtc cagcttggtg tccacgaagt
      121 agtagccggg cagttgcacg ggcttcttgg ccatgtagat ggtcttggac tccaccaggt
      181 tgaggccgcc gtccttcagc ttcaggcgcc ccaggagctc gcccttcagc acgccttctt
      241 cggggtacag gcgctcggtg gcataatccc agcccaaggt cttcttctgc attacggggc
      301 cgtcgggggg gaagttggtg ccgcgcatct tcaccttgta gatcagcgtg ccgtcctgca
      361 gggaggagtc ctgtgtcgcg gtcaccagac cgccgtcctc gaagtgcatc acgcgctccc
      421 acttgaagcc ctcggggaag gacagcttca tgtaatcggg gacgtcggcg gggtgcttca
      481 cgtacatctt ggagccgtac atgatcaggg gggacaggat gtcccaggcg aagggcaggg
      541 ggccgccctt ggtcaccttc agcttggcgg tctgggtgcc ctcgtaaggg cggccctcgc
      601 cctcgccctc gatctcgaac tcgtggccgt tcatggagcc ctccaagcgc accttgaagc
      661 gcatgaactc tttgatgacc tcctcgctct tgctcaccat ggtaccggag ctgccgctgc
      721 cggtgctgcc ggtgccatgc cccagtgccc cggagctgga gatcttcttc agtcttccga
      781 atgctcggac agcatggcct gttttctgcc agcgcctctt catggtggcg aattcc
//
