LOCUS       sequence-101715-        1092 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of human beta-catenin with Flag tag and S33Y 
            mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Kolligs FT, Hu G, Dang CV, Fearon ER
  TITLE     Neoplastic transformation of RK3E by mutant beta-catenin requires 
            deregulation of Tcf/Lef transcription but not activation of c-myc 
            expression.
  JOURNAL   Mol Cell Biol. 1999 Aug . 19(8):5696-706.
  PUBMED    10409758
REFERENCE   2  (bases 1 to 1092)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 1999 Aug . 19(8):5696-706."
COMMENT     Sequence Label: pcDNA3-S33Y Beta-catenin
FEATURES             Location/Qualifiers
     source          1..1092
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             168..203
                     /codon_start=1
                     /product="nanobody epitope tag corresponding to amino acids
                     16-27 of human beta-catenin (Traenkle et al., 2015)"
                     /label=BC2 tag
                     /translation="PDRKAAVSHWQQ"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc ttggtaccga gctcggatcc ccagcgtgga
      121 caatggctac tcaagctgat ttgatggagt tggacatggc catggaacca gacagaaaag
      181 cggctgttag tcactggcag caacagtctt acctggacta tggaatccat tctggtgcca
      241 ctaccacagc tccttctctg agtggtaaag gcaatcctga ggaagaggat gtggatacct
      301 cccaagtcct gtatgagtgg gaacagggat tttctcagtc cttcactcaa gaacaagtag
      361 ctgatattga tggacagtat gcaatgactc gagctcagag ggtacgagct gctatgttcc
      421 ctgagacatt agatgagggc atgcagatcc catctacaca gtttgatgct gctcatccca
      481 ctaatgtcca gcgtttggct gaaccatcac agatgctgaa acatgcagtt gtaaacttga
      541 ttaactatca agatgatgca gaacttgcca cacgtgcaat ccctgaactg acaaaactgc
      601 taaatgacga ggaccaggtg gtggttaata aggctgcagt tatggtccat cagctttcta
      661 aaaaggaagc ttccagacac gctatcatgc gttctcctca gatggtgtct gctattgtac
      721 gtaccatgca gaatacaaat gatgtagaaa cagctcgttg taccgctggg accttgcata
      781 acctttccca tcatcgtgag ggcttactgg ccatctttaa gtctggaggc attcctgccc
      841 tggtgaaaat gcttggttca ccagtggatt ctgtgttgtt ttatgccatt acaactctcc
      901 acaacctttt attacatcaa gaaggagcta aaatggcagt gcgtttagct ggtgggctgc
      961 agaaaatggt tgccttgctc aacaaaacaa atgttaaatt cttggctatt acgacagact
     1021 gccttcaaat tttagcttat ggcaaccaag aaagcaagct catcatactg gctagtggtg
     1081 gaccccaagc tt
//
