LOCUS       sequence-101716-        1078 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of human beta-catenin with Flag tag and S33Y 
            mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Kolligs FT, Hu G, Dang CV, Fearon ER
  TITLE     Neoplastic transformation of RK3E by mutant beta-catenin requires 
            deregulation of Tcf/Lef transcription but not activation of c-myc 
            expression.
  JOURNAL   Mol Cell Biol. 1999 Aug . 19(8):5696-706.
  PUBMED    10409758
REFERENCE   2  (bases 1 to 1078)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 1999 Aug . 19(8):5696-706."
COMMENT     Sequence Label: pcDNA3-S33Y Beta-catenin
FEATURES             Location/Qualifiers
     source          1..1078
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(56..79)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tttaggtgac actatagaat agggccctct agatgcatgc tcgagcggcc gcttacttgt
       61 catcgtcgtc cttgtagtcc aggtcagtat caaaccaggc cagctgattg ctgtcacctg
      121 gaggcagccc atccatgagg tcctgggcat gccccagatc tggcagccca tcaactggat
      181 agtcagcacc agggtggtgg ccacccatct catgttccat catggggtcc atacccaagg
      241 catcctggcc atatccacca gagtgaaaag aacgatagct aggatcatcc tggcgatatc
      301 caaggggttc tccctgggca ccaatatcaa gtccaagatc agcagtctca ttccaagcca
      361 ttggctctgt tctgaagaga gagctggtca gctcaactga aagccgtttc ttgtaatctt
      421 gtggcttgtc ctcagacatt cggaacaaaa cagcagctgc atatgtcgcc acaccttcat
      481 tcctagagtg aagtaactct gtcagaggag ctgtggctcc ctcagcttca atagcttctg
      541 cagcttcctt gtcctgagca agttcacaga ggacccctgc agctactctt tggatgtttt
      601 caatgggaga ataaagcagc tgcacaaaca atggaatggt atttagtcct ctgataacaa
      661 ttcggttgtg aacatcccga gctaggatgt gaagggctcc ggtacaacct tcaactattt
      721 cttccatgcg gaccccctcc acaaattgct gctgtgtccc acccatggac gtacggcgct
      781 gggtatcctg atgtgcacga acaagcaact gaactagtcg tggaatggca ccctgctcac
      841 gcaaaggtgc atgatttgcg ggacaaaggg caagatttcg aatcaatcca acagtagcct
      901 ttatcagagg ccagtgggat ggtgggtgta agagcttaac cacaactggt agtccatagt
      961 gaaggcgaac tgcattctgg gccatctctg cttcttggtg tcggctggtc agatgacgaa
     1021 gagcacagat ggcaggctca gtgatgtctt ccctgtcacc agcccgaagg acagtacg
//
