LOCUS       sequence-10173-e       11894 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11894)
  AUTHORS   Hakre S, Tussie-Luna MI, Ashworth T, Novina CD, Settleman J, Sharp 
            PA, Roy AL
  TITLE     Opposing functions of TFII-I spliced isoforms in growth 
            factor-induced gene expression.
  JOURNAL   Mol Cell. 2006 Oct 20. 24(2):301-8.
  PUBMED    17052463
REFERENCE   2  (bases 1 to 11894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2006 Oct 20. 24(2):301-8."
COMMENT     Sequence Label: pSFG-TFII-I-GFP beta
FEATURES             Location/Qualifiers
     source          1..11894
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             397..988
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    1051..1408
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     primer_bind     1819..1841
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1857..1873
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     2211..2227
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     protein_bind    join(2286..2304,2305..2327,2328..2346,2347..2369,2370..2388,
                     2389..2411,2412..2430,2431..2453,2454..2472,2473..2495,
                     2496..2514,2515..2537,2538..2556)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2589..2627
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2624..2648
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2726..2743
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             2753..2770
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     regulatory      5741..5750
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5747..5749,5750..5752,5753..6466)
                     /codon_start=1
                     /product="enhanced blue variant of GFP"
                     /label=EBFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTHGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5792..5813)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6053..6072)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6400..6421
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(6482..7055,7056..7058,7059..7067)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6649..6666)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6876..6895
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(7068..7688,7689..8075)
                     /codon_start=1
                     /product="tetracycline-controlled transactivator, 
                     comprising a fusion of the tetracycline repressor TetR with
                     the C-terminal activation domain of herpes simplex virus 
                     VP16"
                     /label=tTA
                     /note="In the Tet-Off(R) system, tTA binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGSAYSRARTKNNYGSTI
                     EGLLDLPDDDAPEEAGLAAPRLSFLPAGHTRRLSTAPPTDVSLGDELHLDGEDVAMAHA
                     DALDDFDLDMLGDGDSPGPGFTPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     primer_bind     complement(7141..7160)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     primer_bind     complement(9265..9282)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9265..9281)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9274..9296)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(9490..9509)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9609..9631
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9670..9688)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9756..9860
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9861..9929,9930..10721)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10079..10098)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10892..11480
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11381..11400
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11634..11651
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11768..11789
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11804..11809,11810..11827,11828..11834)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11842..11858
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11847..11869
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11866..11882
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11866..11882
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagctttgct cttaggagtt tcctaataca tcccaaactc aaatatataa agcatttgac
       61 ttgttctatg ccctaggggg cggggggaag ctaagccagc tttttttaac atttaaaatg
      121 ttaattccat tttaaatgca cagatgtttt tatttcataa gggtttcaat gtgcatgaat
      181 gctgcaatat tcctgttacc aaagctagta taaataaaaa tagataaacg tggaaattac
      241 ttagagtttc tgtcattaac gtttccttcc tcagttgaca acataaatgc gctgctgagc
      301 aagccagttt gcatctgtca ggatcaattt cccattatgc cagtcatatt aattactagt
      361 caattagttg atttttattt ttgacatata catgtgaatg aaagacccca cctgtaggtt
      421 tggcaagcta gcttaagtaa cgccattttg caaggcatgg aaaaatacat aactgagaat
      481 agagaagttc agatcaaggt caggaacaga tggaacagct gaatatgggc caaacaggat
      541 atctgtggta agcagttcct gccccgctca gggccaagaa cagatggaac agctgaatat
      601 gggccaaaca ggatatctgt ggtaagcagt tcctgccccg ctcagggcca agaacagatg
      661 gtccccagat gcggtccagc cctcagcagt ttctagagaa ccatcagatg tttccagggt
      721 gccccaagga cctgaaatga ccctgtgcct tatttgaact aaccaatcag ttcgcttctc
      781 gcttctgttc gcgcgcttat gctccccgag ctcaataaaa gagcccacaa cccctcactc
      841 ggggcgccag tcctccgatt gactgagtcg cccgggtacc cgtgtatcca ataaaccctc
      901 ttgcagttgc atccgacttg tggtctcgct gttccttggg agggtctcct ctgagtgatt
      961 gactacccgt cagcgggggt ctttcatttg ggggctcgtc cgggatcggg agacccctgc
     1021 ccagggacca ccgacccacc accgggaggt aagctggcca gcaacttatc tgtgtctgtc
     1081 cgattgtcta gtgtctatga ctgattttat gcgcctgcgt cggtactagt tagctaacta
     1141 gctctgtatc tggcggaccc gtggtggaac tgacgagttc ggaacacccg gccgcaaccc
     1201 tgggagacgt cccagggact tcgggggccg tttttgtggc ccgacctgag tcctaaaatc
     1261 ccgatcgttt aggactcttt ggtgcacccc ccttagagga gggatatgtg gttctggtag
     1321 gagacgagaa cctaaaacag ttcccgcctc cgtctgaatt tttgctttcg gtttgggacc
     1381 gaagccgcgc cgcgcgtctt gtctgctgca gcatcgttct gtgttgtctc tgtctgactg
     1441 tgtttctgta tttgtctgaa aatatgggcc cgggctagcc tgttaccact cccttaagtt
     1501 tgaccttagg tcactggaaa gatgtcgagc ggatcgctca caaccagtcg gtagatgtca
     1561 agaagagacg ttgggttacc ttctgctctg cagaatggcc aacctttaac gtcggatggc
     1621 cgcgagacgg cacctttaac cgagacctca tcacccaggt taagatcaag gtcttttcac
     1681 ctggcccgca tggacaccca gaccaggtcc cctacatcgt gacctgggaa gccttggctt
     1741 ttgacccccc tccctgggtc aagccctttg tacaccctaa gcctccgcct cctcttcctc
     1801 catccgcccc gtctctcccc cttgaacctc ctcgttcgac cccgcctcga tcctcccttt
     1861 atccagccct cactccttct ctaggcgccc ccatatggcc atatgagatc ttatatgggg
     1921 cacccccgcc ccttgtaaac ttccctgacc ctgacatgac aagagttact aacagcccct
     1981 ctctccaagc tcacttacag gctctctact tagtccagca cgaagtctgg agacctctgg
     2041 cggcagccta ccaagaacaa ctggaccgac cggtggtacc tcacccttac cgagtcggcg
     2101 acacagtgtg ggtccgccga caccagacta agaacctaga acctcgctgg aaaggacctt
     2161 acacagtcct gctgaccacc cccaccgccc tcaaagtaga cggcatcgca gcttggatac
     2221 acgccgccca cgtgaaggct gccgaccccg ggggtggacc atcctctagc cctcgagttt
     2281 accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga
     2341 tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc
     2401 gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat
     2461 cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg
     2521 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gctcggtacc
     2581 cgggtcgagg taggcgtgta cggtgggagg cctatataag cagagctcgt ttagtgaacc
     2641 gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga caccgggacc
     2701 gatccgacct gccatgggca gcagccatca tcatcatcat cacagcagcg gcctggtgcc
     2761 gcgcggcagc catatggccc aagttgcaat gtccaccctc cccgttgaag atgaggagtc
     2821 ctcggagagc aggatggtgg tgacattcct catgtcagct ctcgagtcca tgtgtaaaga
     2881 actggccaag tccaaagccg aagtggcctg cattgcagtg tatgaaacag acgtgtttgt
     2941 cgtcggaact gaaagaggac gtgcttttgt caataccaga aaggattttc aaaaagattt
     3001 tgtaaaatat tgtgttgaag aagaagaaaa agctgcagag atgcataaaa tgaaatctac
     3061 aacccaggca aatcggatga gtgtagatgc tgtagaaatt gaaacactca gaaaaacagt
     3121 tgaggactat ttctgctttt gctatgggaa agctttaggc aaatccacag tggtacctgt
     3181 accatatgag aagatgctgc gagaccagtc ggctgtggta gtgcaggggc ttccggaagg
     3241 tgttgccttt aaacaccccg agaactatga tcttgcaacc ctgaaatgga ttttggagaa
     3301 caaagcagga atttcattca tcattaagag acctttttta gagccaaaga agcatgtagg
     3361 tggtcgtgtg atggtaacag atgctgacag gtcaatacta tctccaggtg gaagttgtgg
     3421 ccccatcaaa gtgaaaactg aacccacaga agattctggc atttccctgg aaatggcagc
     3481 tgtgacagta aaggaagaat cagaagatcc tgattattat caatataaca ttcaaggaag
     3541 ccaccattct tcagagggca atgaaggcac agaaatggaa gtaccagcag aagattctac
     3601 tcaacatgtc ccttcagaaa caagtgagga ccctgaagtt gaggtgacta ttgaagatga
     3661 tgattattct ccaccgtcta agagaccaaa ggccaatgag ctaccgcagc caccagtccc
     3721 ggaacccgcc aatgctggga agcggaaagt gagggagttc aacttcgaga aatggaatgc
     3781 tcgcatcact gatctacgta aacaagttga agaattgttt gaaaggaaat atgctcaagc
     3841 cataaaagcc aaaggtccgg tgacgatccc gtaccctctt ttccagtctc atgttgaaga
     3901 tctttatgta gaaggacttc ctgaaggaat tccttttaaa aggccatcta cttacggaat
     3961 tcctcgcctg gagaggatat tacttgcaaa ggaaaggatt cgttttgtga ttaagaaaca
     4021 tgagcttctg aattcaacac gtgaagattt acagcttgat aagccagctt caggagtaaa
     4081 ggaagaatgg tatgccagaa tcactaaatt aagaaagatg gtggatcagc ttttctgcaa
     4141 aaaatttgcg gaacgcttgg ggagcactga agccaaggct gtaccgtacc aaaaatttga
     4201 ggcacacccg aatgatctgt acgtggaagg actgccagaa aacattcctt tccgaagtcc
     4261 ctcatggtat ggaatcccaa ggctggaaaa aatcattcaa gtgggcaatc gaattaaatt
     4321 tgttattaaa agaccagaac ttctgactca cagtaccact gaagttactc agccaagaac
     4381 gaatacacca gtcaaagaag attggaatgt cagaattacc aagctacgga agcaagtgga
     4441 agagattttt aatttgaaat ttgctcaagc tcttggactc accgaggcag taaaagtacc
     4501 atatcctgtg tttgaatcaa acccggagtt cttgtatgtg gaaggcttgc cagaggggat
     4561 tcccttccga agccctacct ggtttggaat tccacgactt gaaaggatcg tccgcgggag
     4621 taataaaatc aagttcgttg ttaaaaaacc tgaactagtt atttcctact tgcctcctgg
     4681 gatggctagt aaaataaaca ctaaagcttt gcagtccccc aaaagaccac gaagtcctgg
     4741 gagtaattca aaggttcctg aaattgaggt caccgtggaa ggccctaata acaacaatcc
     4801 tcaaacctca gctgttcgaa ccccgaccca gactaacggt tctaacgttc ccttcaagcc
     4861 acgagggaga gagttttcct ttgaggcctg gaatgccaaa atcacggacc taaaacagaa
     4921 agttgaaaat ctcttcaatg agaaatgtgg ggaagctctt ggccttaaac aagctgtgaa
     4981 ggtgccgttc gcgttatttg agtctttccc ggaagacttt tatgtggaag gcttacctga
     5041 gggtgtgcca ttccgaagac catcgacttt tggcattccg agactggaga agatactcag
     5101 aaacaaagcc aaaattaagt tcatcattaa aaagcccgaa atgtttgaga cggcgattaa
     5161 ggagagcacc tcctctaaga gccctcccag aaaaataaat tcatcaccca atgttaatac
     5221 tactgcatca ggtgttgaag accttaacat cattcaggtg acaattccag atgatgataa
     5281 tgaaagactc tcgaaagttg aaaaagctag acagctaaga gaacaagtga atgacctctt
     5341 tagtcggaaa tttggtgaag ctattggtat gggttttcct gtgaaagttc cctacaggaa
     5401 aatcacaatt aaccctggct gtgtggtggt tgatggcatg cccccggggg tgtccttcaa
     5461 agcccccagc tacctggaaa tcagctccat gagaaggatc ttagactctg ccgagtttat
     5521 caaattcacg gtcattagac catttccagg acttgtgatt aataaccagc tggttgatca
     5581 gagtgagtca gaaggccccg tgatacaaga atcagctgaa ccaagccagt tggaagttcc
     5641 agccacagaa gaaataaaag agactgatgg aagctctcag atcaagcaag aaccagaccc
     5701 cacgtggtcg acggtaccgc gggcccggga tccaccggtc gccaccatgg tgagcaaggg
     5761 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
     5821 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
     5881 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct
     5941 gacccacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt
     6001 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
     6061 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
     6121 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
     6181 cttcaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa
     6241 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
     6301 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca
     6361 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
     6421 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaaagcg gccgcggatc
     6481 ccccctctcc ctcccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt
     6541 gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc
     6601 ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag
     6661 gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac
     6721 aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc
     6781 tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc
     6841 acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca
     6901 aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt
     6961 gcacatgctt tacatgtgtt tagtcgaggt taaaaaacgt ctaggccccc cgaaccacgg
     7021 ggacgtggtt ttcctttgaa aaacacgatg ataagcttgc cacaaccatg tctagattag
     7081 ataaaagtaa agtgattaac agcgcattag agctgcttaa tgaggtcgga atcgaaggtt
     7141 taacaacccg taaactcgcc cagaagctag gtgtagagca gcctacattg tattggcatg
     7201 taaaaaataa gcgggctttg ctcgacgcct tagccattga gatgttagat aggcaccata
     7261 ctcacttttg ccctttagaa ggggaaagct ggcaagattt tttacgtaat aacgctaaaa
     7321 gttttagatg tgctttacta agtcatcgcg atggagcaaa agtacattta ggtacacggc
     7381 ctacagaaaa acagtatgaa actctcgaaa atcaattagc ctttttatgc caacaaggtt
     7441 tttcactaga gaatgcatta tatgcactca gcgctgtggg gcattttact ttaggttgcg
     7501 tattggaaga tcaagagcat caagtcgcta aagaagaaag ggaaacacct actactgata
     7561 gtatgccgcc attattacga caagctatcg aattatttga tcaccaaggt gcagagccag
     7621 ccttcttatt cggccttgaa ttgatcatat gcggattaga aaaacaactt aaatgtgaaa
     7681 gtgggtccgc gtacagccgc gcgcgtacga aaaacaatta cgggtctacc atcgagggcc
     7741 tgctcgatct cccggacgac gacgcccccg aagaggcggg gctggcggct ccgcgcctgt
     7801 cctttctccc cgcgggacac acgcgcagac tgtcgacggc ccccccgacc gatgtcagcc
     7861 tgggggacga gctccactta gacggcgagg acgtggcgat ggcgcatgcc gacgcgctag
     7921 acgatttcga tctggacatg ttgggggacg gggattcccc gggtccggga tttacccccc
     7981 acgactccgc cccctacggc gctctggata tggccgactt cgagtttgag cagatgttta
     8041 ccgatgccct tggaattgac gagtacggtg ggtagggggc gcgaagatcc ggattagtcc
     8101 aatttgttaa agacaggata tcagtggtcc aggctctagt tttgactcaa caatatcacc
     8161 agctgaagcc tatagagtac gagccataga taaaataaaa gattttattt agtctccaga
     8221 aaaagggggg aatgaaagac cccacctgta ggtttggcaa gctagctcga ccctagagaa
     8281 ccatcagatg tttccagggt gccccaagga cctgaaatga ccctgtgcct tatttgaact
     8341 aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct gctccccgag ctcaataaaa
     8401 gagcccacaa cccctcactc ggggcgccag tcctccgatt gactgagtcg cccgggtacc
     8461 cgtgtatcca ataaaccctc ttgcagttgc atccgacttg tggtctcgct gttccttggg
     8521 agggtctcct ctgagtgatt gactacccgt cagcgggggt ctttcacaca tgcagcatgt
     8581 atcaaaatta atttggtttt ttttcttaag tatttacatt aaatggccat agtacttaaa
     8641 gttacattgg cttccttgaa ataaacatgg agtattcaga atgtgtcata aatatttcta
     8701 attttaagat agtatctcca ttggctttct actttttctt ttattttttt ttgtcctctg
     8761 tcttccattt gttgttgttg ttgtttgttt gtttgtttgt tggttggttg gttaattttt
     8821 ttttaaagat cctacactat agttcaagct agactattag ctactctgta acccagggtg
     8881 accttgaagt catgggtagc ctgctgtttt agccttccca catctaagat tacaggtatg
     8941 agctatcatt tttggtatat tgattgattg attgattgat gtgtgtgtgt gtgattgtgt
     9001 ttgtgtgtgt gactgtgaaa atgtgtgtat gggtgtgtgt gaatgtgtgt atgtatgtgt
     9061 gtgtgtgagt gtgtgtgtgt gtgtgtgcat gtgtgtgtgt gtgactgtgt ctatgtgtat
     9121 gactgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt tgtgaaaaaa
     9181 tattctatgg tagtgagagc caacgctccg gctcaggtgt caggttggtt tttgagacag
     9241 agtctttcac ttagcttgga attcactggc cgtcgtttta caacgtcgtg actgggaaaa
     9301 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     9361 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     9421 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg
     9481 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
     9541 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
     9601 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgat
     9661 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     9721 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     9781 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     9841 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     9901 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     9961 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
    10021 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
    10081 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
    10141 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
    10201 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
    10261 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
    10321 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
    10381 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
    10441 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
    10501 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
    10561 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
    10621 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
    10681 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
    10741 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
    10801 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
    10861 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
    10921 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
    10981 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
    11041 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
    11101 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
    11161 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
    11221 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
    11281 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
    11341 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
    11401 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
    11461 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
    11521 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
    11581 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
    11641 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
    11701 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
    11761 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
    11821 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
    11881 accatgatta cgcc
//
