LOCUS       sequence-101737-        1079 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of Sec61 beta fused to mCherry, used as a general ER 
            marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Zurek N, Sparks L, Voeltz G
  TITLE     Reticulon short hairpin transmembrane domains are used to shape ER 
            tubules.
  JOURNAL   Traffic. 2011 Jan;12(1):28-41. doi: 
            10.1111/j.1600-0854.2010.01134.x. Epub 2010 Nov 12.
  PUBMED    20955502
REFERENCE   2  (bases 1 to 1079)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/j.1600-0854.2010.01134.x"; journalName: "Traffic"; date: 
            "2011-01"; volume: "12"; issue: "1"; pages: "28-41"
COMMENT     Sequence Label: mCh-Sec61 beta
FEATURES             Location/Qualifiers
     source          1..1079
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             45..752
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(190..208)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     458..477
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             759..1049
                     /codon_start=1
                     /gene="SEC61B"
                     /product="human Sec61-beta subunit of the translocon in the
                     endoplasmic reticulum"
                     /label=Sec61-beta
                     /translation="MPGPTPSGTNVGSSGRSPSKAVAARAAGSTVRQRKNASCGTRSAG
                     RTTSAGTGGMWRFYTEDSPGLKVGPVPVLVMSLLFIASVFMLHIWGKYTRS"
ORIGIN
        1 ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcatggtg agcaagggcg
       61 aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct
      121 ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca
      181 cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc
      241 tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg
      301 actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg
      361 acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca
      421 aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca
      481 tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga
      541 tcaagcagag gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct
      601 acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca
      661 tcacctccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc
      721 actccaccgg cggcatggac gagctgtaca agagatctat gcctggtccg acccccagtg
      781 gcactaacgt gggatcctca gggcgctctc ccagcaaagc agtggccgcc cgggcggcgg
      841 gatccactgt ccggcagagg aaaaatgcca gctgtgggac aaggagtgca ggccgcacaa
      901 cctcggcagg caccgggggg atgtggcgat tctacacaga agattcacct gggctcaaag
      961 ttggccctgt tccagtattg gttatgagtc ttctgttcat cgcttctgta tttatgttgc
     1021 acatttgggg caagtacact cgttcgtagg aattctgcag tcgacggtac cgcgggccc
//
