LOCUS       sequence-101801-        1000 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses PTDH mutant Opt12.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   McLachlan MJ, Johannes TW, Zhao H
  TITLE     Further improvement of phosphite dehydrogenase thermostability by 
            saturation mutagenesis.
  JOURNAL   Biotechnol Bioeng. 2008 Feb 1;99(2):268-74.
  PUBMED    17615560
REFERENCE   2  (bases 1 to 1000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng."; date: "2008-02-1"; volume: "99(2)"; pages: "268-74"
COMMENT     Sequence Label: pET15b-Opt12
FEATURES             Location/Qualifiers
     source          1..1000
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ggctttgtta gcagccggat ccttatcagt ctgcggcagg attggccttg ggcagacggt
       61 tcacagcgtt gattgggcgc tcacctgcca atgcctggag gatgttctgc gctgcacaac
      121 gttcaatctc caggcgcacc gcgcgcactg ccgaccctat gtgcggagtg aacagcgtat
      181 tcggatgcgc gagcagcgca ggatcgatgc acagcggccg gtccgcgcga gcccagtctt
      241 ccatttcgaa tacatccgcc gcatacccgc cgagctggcc tcgctcaagc gccgcgagca
      301 cggcggcttc atccactacc gagccacgac aggggtttac aagcagagcg cccggccgta
      361 cgagggcaag cagctcggcg ttgaccagat gcagggtatc ggcattcaag ggaagcgcca
      421 gcaggatgaa gtccgagctg gcgaagagtt cgctgcacgc cacctggcgc aggccgagcc
      481 gttgctcggt ttgtgtatcc agagccttcg ccgcgtggta ctgcagggtc gcgccccatc
      541 cctgcaagcg atcagccatg gccagtccga tggcgcccat gccaaggaag ccgaccgtag
      601 cgttatccag ccccgtgccg tagaaatgtg gttgccagcc cttgaacttg ccagagcgga
      661 cgaacgcatc tgctgcccgc agatgccgcc ccagccccac cgccagtccg atcgccagct
      721 cggcagtcgg gaccgtcaac agatcaggca cgaaggtcag ccagaccccg cgggcagtac
      781 aggcgtccac atcgaaattg tcgaagccct tgagcgcgca gccgattaca cgcagctcag
      841 ggcaggcttg aagaaagtct gcatcgaccc gatcgggcat gaacgccatc atcgcctgag
      901 catcgcgaca gcggcgcaga atttcctcgc gcgtcagcgt gctgtcggtc tggttggtta
      961 tcagctcgca atgtggcgcc agcagttgca ggatctcttc
//
