LOCUS       sequence-101832-         747 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 747)
  TITLE     SGC Toronto Q2 2014
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 4QQM
FEATURES             Location/Qualifiers
     source          1..747
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     8..27
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8..26
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    27..51
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             66..88
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             98..115
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             128..148
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             712..729
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gcgaaattaa tacgactcac tataggggaa ttgtgagcgg ataacaattc ccctctagaa
       61 ataattttgt ttaactttaa gaaggagata taccatgcat catcatcatc atcacagcag
      121 cggcagagaa aacttgtatt tccagggctc agctctgccg gccaccctag actccctgtc
      181 catccaccag ctcgcagcac agggggagct ggaccagctg aaggagcatt tgcggaaagg
      241 tgacaacctc gtcaacaagc cagacgagcg cggcttcacc cccctcatct gggcctccgc
      301 ctttggagag attgagaccg ttcgcttcct gctggagtgg ggtgccgacc cccacatcct
      361 ggcaaaagag cgagagagcg ccctgtcgct ggccagcaca ggcggctaca cagacattgt
      421 ggggctgctg ctggagcgtg acgtggacat caacatctat gattggaatg gagggacgcc
      481 actgctgtac gctgtgcgcg ggaaccacgt gaaatgcgtt gaggccttgc tggcccgagg
      541 cgctgacctc accaccgaag ccgactctgg ctacaccccg atggaccttg ccgtggccct
      601 gggataccgg aaagtgcaac aggtgatcga gaaccacatc ctcaagctct tccagagcaa
      661 cctggtgccc gctgaccctg agtgatgacg aagattgcgg ccgcactcga gcaccaccac
      721 caccaccact gagatccggc tgctaac
//
