LOCUS       sequence-101865-        7830 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Self-inactivating retrovirus vector containing the DsRed-Express2 
            gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7830)
  AUTHORS   Fujita T, Fujii H
  TITLE     Identification of Proteins Associated with an IFNgamma-Responsive 
            Promoter by a Retroviral Expression System for enChIP Using CRISPR.
  JOURNAL   PLoS One. 2014 Jul 22;9(7):e103084. doi: 
            10.1371/journal.pone.0103084. eCollection 2014.
  PUBMED    25051498
REFERENCE   2  (bases 1 to 7830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-07-22"; volume: "9(7):e103084. doi"; pages: " 
            10.1371/journal.pone.0103084. eCollection 2014"
COMMENT     Sequence Label: pSIR-DsRed-Express2
FEATURES             Location/Qualifiers
     source          1..7830
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    580..937
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             996..1412
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1342..1364
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1380..1396
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             2815..3492
                     /codon_start=1
                     /product="noncytotoxic tetrameric variant of DsRed 
                     fluorescent protein (Strack et al., 2008)"
                     /label=DsRed-Express2
                     /note="mammalian codon-optimized"
                     /translation="MDSTENVIKPFMRFKVHMEGSVNGHEFEIEGEGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGTFIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERLYPRDGVLKGEIHK
                     ALKLKGGGHYLVEFKSIYMAKKPVKLPGYYYVDSKLDITSHNEDYTVVEQYERAEARHH
                     LFQ"
     primer_bind     complement(2995..3015)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     3213..3232
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     3407..3430
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(4814..4830)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4814..4830)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4827..4849)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4838..4854
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4862..4868,4869..4886,4887..4892))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4907..4928
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5045..5062)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5216..5804)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5296..5315)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5975..6766,6767..6835))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6598..6617
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6836..6940)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7008..7026
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7064..7086)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7186..7205
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7399..7421
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7413..7430
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7414..7430
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat
       61 ggaaaaatac ataactgaga atagagaagt tcagatcaag gtcaggaaca gatggaacag
      121 ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
      181 gaacagatgg tccccagatg cggtccagcc ctcagcagtt tctagagaac catcagatgt
      241 ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta accaatcagt
      301 tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag agcccacaac
      361 ccctcactcg gggcgccagt cctccgattg actgagtcgc ccgggtaccc gtgtatccaa
      421 taaaccctct tgcagttgca tccgacttgt ggtctcgctg ttccttggga gggtctcctc
      481 tgagtgattg actacccgtc agcgggggtc tttcatttgg gggctcgtcc gggatcggga
      541 gacccctgcc cagggaccac cgacccacca ccgggaggta agctggccag caacttatct
      601 gtgtctgtcc gattgtctag tgtctatgac tgattttatg cgcctgcgtc ggtactagtt
      661 agctaactag ctctgtatct ggcggacccg tggtggaact gacgagttcg gaacacccgg
      721 ccgcaaccct gggagacgtc ccagggactt cgggggccgt ttttgtggcc cgacctgagt
      781 ccaaaaatcc cgatcgtttt ggactctttg gtgcaccccc cttagaggag ggatatgtgg
      841 ttctggtagg agacgagaac ctaaaacagt tcccgcctcc gtctgaattt ttgctttcgg
      901 tttgggaccg aagccgcgcc gcgcgtcttg tctgctgcag catcgttctg tgttgtctct
      961 gtctgactgt gtttctgtat ttgtctgaga atatgggcca gactgttacc actcccttaa
     1021 gtttgacctt aggtcactgg aaagatgtcg agcggatcgc tcacaaccag tcggtagatg
     1081 tcaagaagag acgttgggtt accttctgct ctgcagaatg gccaaccttt aacgtcggat
     1141 ggccgcgaga cggcaccttt aaccgagacc tcatcaccca ggttaagatc aaggtctttt
     1201 cacctggccc gcatggacac ccagaccagg tcccctacat cgtgacctgg gaagccttgg
     1261 cttttgaccc ccctccctgg gtcaagccct ttgtacaccc taagcctccg cctcctcttc
     1321 ctccatccgc cccgtctctc ccccttgaac ctcctcgttc gaccccgcct cgatcctccc
     1381 tttatccagc cctcactcct tctctaggcg ccaaacctaa acctcaagtt ctttctgaca
     1441 gtggggggcc gctcatcgac ctacttacag aagacccccc gccttatagg gacccaagac
     1501 cacccccttc cgacagggac ggaaatggtg gagaagcgac ccctgcggga gaggcaccgg
     1561 acccctcccc aatggcatct cgcctacgtg ggagacggga gccccctgtg gccgactcca
     1621 ctacctcgca ggcattcccc ctccgcgcag gaggaaacgg acagcttcaa tactggccgt
     1681 tctcctcttc tgacctttac aactggaaaa ataataaccc ttctttttct gaagatccag
     1741 gtaaactgac agctctgatc gagtctgttc tcatcaccca tcagcccacc tgggacgact
     1801 gtcagcagct gttggggact ctgctgaccg gagaagaaaa acaacgggtg ctcttagagg
     1861 ctagaaaggc ggtgcggggc gatgatgggc gccccactca actgcccaat gaagtcgatg
     1921 ccgcttttcc cctcgagaag cttgtcgacg gatccgaatt cgtcgaggga agaaataata
     1981 aagacattaa aatcactttt gtaaactttt taaataaggg aaattgatta aaacaacgcg
     2041 aagttgtaaa atgccatata ctgcatacat tttgtgggaa aaaatgtgca tttacctgct
     2101 caccaaatta tcttattagc tagcagtaga gctctttgtg tgatgggaag atgggataac
     2161 agctgttgtt actgtactgg tttttttttt taatatattt aaaaaaaaga gtgtctccta
     2221 aagtgtaagc gctatcatgc tggactaatg ttttaattta atttaattgt tgtcttggta
     2281 gagatggggt ctcgctattt tgctcacgca gatctcaaac tcctgacgcc caggtacact
     2341 cccatctcgg cctcctaaaa tggtgggatc acagacgtca gccaccgagc ccggtcactt
     2401 ttttgtattc cccacagtat tgatgtatat cttctgcgtt caaaagcaat tttttaaagc
     2461 ctcataacgt ggtaacagaa tactttgcac attacaaaat tcagaacacg gaaacaagaa
     2521 gctcgctttt ttttcccccc tatttcggtt tggcccttta gatttcccct cccccaccgg
     2581 ggcgggactt cccgccgact tctttcaggt tctcagttcg gtccgccaac tgtcgtataa
     2641 aggcgctgcc tcaggccaga ggcctcacaa agcgcgtcga gacagcaagc gaaccggaat
     2701 tgccagctgg ggcgccctct ggtaaggttg ggaagccctg caaagtaaac tggatggctt
     2761 tcttgccgcc aaggatctga tggcgcaggg gatcaagatc gatctgatcc aaccatggat
     2821 agcactgaga acgtcatcaa gcccttcatg cgcttcaagg tgcacatgga gggctccgtg
     2881 aacggccacg agttcgagat cgagggcgag ggcgagggca agccctacga gggcacccag
     2941 accgccaagc tgcaggtgac caagggcggc cccctgccct tcgcctggga catcctgtcc
     3001 ccccagttcc agtacggctc caaggtgtac gtgaagcacc ccgccgacat ccccgactac
     3061 aagaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc
     3121 ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcaccttcat ctaccacgtg
     3181 aagttcatcg gcgtgaactt cccctccgac ggccccgtaa tgcagaagaa gactctgggc
     3241 tgggagccct ccaccgagcg cctgtacccc cgcgacggcg tgctgaaggg cgagatccac
     3301 aaggcgctga agctgaaggg cggcggccac tacctggtgg agttcaagtc aatctacatg
     3361 gccaagaagc ccgtgaagct gcccggctac tactacgtgg actccaagct ggacatcacc
     3421 tcccacaacg aggactacac cgtggtggag cagtacgagc gcgccgaggc ccgccaccac
     3481 ctgttccagt agatggctgg atgatcctcc agcgcgggga tctcatgctg gagatcccgc
     3541 gattagtcca atttgttaaa gacaggatat cagtggtcca ggctctagtt ttgactcaac
     3601 aatatcacca gctgaagcct atagagtacg agccatagat aaaataaaag attttattta
     3661 gtctccagaa aaagggggga atgaaagacc ccacctgtag gtttggcaag ctagcttaag
     3721 taacgccatt ttgcaaggca tggaaaaata cataactgag aatagagaag ttcagatcaa
     3781 ggtcaggaac agatggaaca ggtcgaccta gagaaccatc agatgtttcc agggtgcccc
     3841 aaggacctga aatgaccctg tgccttattt gaactaacca atcagttcgc ttctcgcttc
     3901 tgttcgcgcg cttctgctcc ccgagctcaa taaaagagcc cacaacccct cactcggggc
     3961 gccagtcctc cgattgactg agtcgcccgg gtacccgtgt atccaataaa ccctcttgca
     4021 gttgcatccg acttgtggtc tcgctgttcc ttgggagggt ctcctctgag tgattgacta
     4081 cccgtcagcg ggggtctctt cacatgcagc atgtatcaaa attaatttgg tttttttttc
     4141 ttaagtattt acattaaatg gcccatagta cttaaagtaa catgggcttc cttgaaataa
     4201 acatggagta ttcagaatgt gtcataaata tttctaattt taagatagta tctccattgg
     4261 ctatctactt tttcttatat ttttttttgt cctctgtctt ccattcgttg ttgttgttgt
     4321 ttgtttgttt gtttgttggt tggttggtta attttttttt aaagatccta cactatagtt
     4381 caagctagac tattagctac tctgtaaccc agggtgacct ttgaagtcat gggtagcctg
     4441 ctgttttagc cttcccacat ctaagattac aggtatgagc tatcattttt ggtatattga
     4501 ttgattgatt gattgatgtg tgtgtgtgtg attgtgtttg tgtgtgtgat tgtgtatatg
     4561 tgtgtatggt tgtgtgtgat tgtgtgtatg tatgtttgtg tgtgattgtg tgtgtgtgat
     4621 tgtgcatgtg tgtgtgtgtg attgtgttta tgtgtatgat tgtgtgtgtg tgtgtgtgtg
     4681 tgtgtgtgtg tgtgtgtgtg tgtgtgttgt gtatatatat ttatggtagt gagaggcaac
     4741 gctccggctc aggtgtcagg ttggtttttg agacagagtc tttcgcttag cttggaatta
     4801 attcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     4861 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     4921 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     4981 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     5041 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     5101 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     5161 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     5221 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     5281 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     5341 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     5401 cgctttctca atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     5461 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     5521 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     5581 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     5641 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     5701 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     5761 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     5821 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     5881 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     5941 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     6001 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     6061 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     6121 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     6181 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     6241 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     6301 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     6361 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     6421 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     6481 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     6541 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     6601 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     6661 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     6721 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     6781 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     6841 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     6901 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     6961 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
     7021 cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
     7081 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
     7141 gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga
     7201 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
     7261 accgcatcag gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
     7321 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
     7381 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gccaagctag
     7441 ctttgctctt aggagtttcc taatacatcc caaactcaaa tatataaagc atttgacttg
     7501 ttctatgccc tagggggcgg ggggaagcta agccagcttt ttttaacatt taaaatgtta
     7561 attccatttt aaatgcacag atgtttttat ttcataaggg tttcaatgtg catgaatgct
     7621 gcaatattcc tgttaccaaa gctagtataa ataaaaatag ataaacgtgg aaattactta
     7681 gagtttctgt cattaacgtt tccttcctca gttgacaaca taaatgcgct gctgagaagc
     7741 cagtttgcat ctgtcaggat caatttccca ttatgccagt catattaatt actagtcaat
     7801 tagttgattt ttatttttga catatacatg
//
