LOCUS       sequence-101867-        8265 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Self-inactivating retrovirus vector containing the human CD2 gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8265)
  AUTHORS   Fujita T, Fujii H
  TITLE     Identification of Proteins Associated with an IFNgamma-Responsive 
            Promoter by a Retroviral Expression System for enChIP Using CRISPR.
  JOURNAL   PLoS One. 2014 Jul 22;9(7):e103084. doi: 
            10.1371/journal.pone.0103084. eCollection 2014.
  PUBMED    25051498
REFERENCE   2  (bases 1 to 8265)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-07-22"; volume: "9(7):e103084. doi"; pages: " 
            10.1371/journal.pone.0103084. eCollection 2014"
COMMENT     Sequence Label: pSIR-hCD2
FEATURES             Location/Qualifiers
     source          1..8265
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    580..937
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             996..1412
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1342..1364
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1380..1396
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(5249..5265)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5249..5265)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5262..5284)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5273..5289
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5297..5303,5304..5321,5322..5327))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5342..5363
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5480..5497)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5651..6239)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5731..5750)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6410..7201,7202..7270))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7033..7052
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7271..7375)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7443..7461
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7499..7521)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7621..7640
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7834..7856
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7848..7865
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7849..7865
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat
       61 ggaaaaatac ataactgaga atagagaagt tcagatcaag gtcaggaaca gatggaacag
      121 ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
      181 gaacagatgg tccccagatg cggtccagcc ctcagcagtt tctagagaac catcagatgt
      241 ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta accaatcagt
      301 tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag agcccacaac
      361 ccctcactcg gggcgccagt cctccgattg actgagtcgc ccgggtaccc gtgtatccaa
      421 taaaccctct tgcagttgca tccgacttgt ggtctcgctg ttccttggga gggtctcctc
      481 tgagtgattg actacccgtc agcgggggtc tttcatttgg gggctcgtcc gggatcggga
      541 gacccctgcc cagggaccac cgacccacca ccgggaggta agctggccag caacttatct
      601 gtgtctgtcc gattgtctag tgtctatgac tgattttatg cgcctgcgtc ggtactagtt
      661 agctaactag ctctgtatct ggcggacccg tggtggaact gacgagttcg gaacacccgg
      721 ccgcaaccct gggagacgtc ccagggactt cgggggccgt ttttgtggcc cgacctgagt
      781 ccaaaaatcc cgatcgtttt ggactctttg gtgcaccccc cttagaggag ggatatgtgg
      841 ttctggtagg agacgagaac ctaaaacagt tcccgcctcc gtctgaattt ttgctttcgg
      901 tttgggaccg aagccgcgcc gcgcgtcttg tctgctgcag catcgttctg tgttgtctct
      961 gtctgactgt gtttctgtat ttgtctgaga atatgggcca gactgttacc actcccttaa
     1021 gtttgacctt aggtcactgg aaagatgtcg agcggatcgc tcacaaccag tcggtagatg
     1081 tcaagaagag acgttgggtt accttctgct ctgcagaatg gccaaccttt aacgtcggat
     1141 ggccgcgaga cggcaccttt aaccgagacc tcatcaccca ggttaagatc aaggtctttt
     1201 cacctggccc gcatggacac ccagaccagg tcccctacat cgtgacctgg gaagccttgg
     1261 cttttgaccc ccctccctgg gtcaagccct ttgtacaccc taagcctccg cctcctcttc
     1321 ctccatccgc cccgtctctc ccccttgaac ctcctcgttc gaccccgcct cgatcctccc
     1381 tttatccagc cctcactcct tctctaggcg ccaaacctaa acctcaagtt ctttctgaca
     1441 gtggggggcc gctcatcgac ctacttacag aagacccccc gccttatagg gacccaagac
     1501 cacccccttc cgacagggac ggaaatggtg gagaagcgac ccctgcggga gaggcaccgg
     1561 acccctcccc aatggcatct cgcctacgtg ggagacggga gccccctgtg gccgactcca
     1621 ctacctcgca ggcattcccc ctccgcgcag gaggaaacgg acagcttcaa tactggccgt
     1681 tctcctcttc tgacctttac aactggaaaa ataataaccc ttctttttct gaagatccag
     1741 gtaaactgac agctctgatc gagtctgttc tcatcaccca tcagcccacc tgggacgact
     1801 gtcagcagct gttggggact ctgctgaccg gagaagaaaa acaacgggtg ctcttagagg
     1861 ctagaaaggc ggtgcggggc gatgatgggc gccccactca actgcccaat gaagtcgatg
     1921 ccgcttttcc cctcgagaag cttgtcgacg gatccgaatt cgtcgaggga agaaataata
     1981 aagacattaa aatcactttt gtaaactttt taaataaggg aaattgatta aaacaacgcg
     2041 aagttgtaaa atgccatata ctgcatacat tttgtgggaa aaaatgtgca tttacctgct
     2101 caccaaatta tcttattagc tagcagtaga gctctttgtg tgatgggaag atgggataac
     2161 agctgttgtt actgtactgg tttttttttt taatatattt aaaaaaaaga gtgtctccta
     2221 aagtgtaagc gctatcatgc tggactaatg ttttaattta atttaattgt tgtcttggta
     2281 gagatggggt ctcgctattt tgctcacgca gatctcaaac tcctgacgcc caggtacact
     2341 cccatctcgg cctcctaaaa tggtgggatc acagacgtca gccaccgagc ccggtcactt
     2401 ttttgtattc cccacagtat tgatgtatat cttctgcgtt caaaagcaat tttttaaagc
     2461 ctcataacgt ggtaacagaa tactttgcac attacaaaat tcagaacacg gaaacaagaa
     2521 gctcgctttt ttttcccccc tatttcggtt tggcccttta gatttcccct cccccaccgg
     2581 ggcgggactt cccgccgact tctttcaggt tctcagttcg gtccgccaac tgtcgtataa
     2641 aggcgctgcc tcaggccaga ggcctcacaa agcgcgtcga gacagcaagc gaaccggaat
     2701 tgccagctgg ggcgccctct ggtaaggttg ggaagccctg caaagtaaac tggatggctt
     2761 tcttgccgcc aaggatctga tggcgcaggg gatcaagatc gatctgatca attcctaaga
     2821 tgagctttcc atgtaaattt gtagccagct tccttctgat tttcaatgtt tcttccaaag
     2881 gtgcagtctc caaagagatt acgaatgcct tggaaacctg gggtgccttg ggtcaggaca
     2941 tcaacttgga cattcctagt tttcaaatga gtgatgatat tgacgatata aaatgggaaa
     3001 aaacttcaga caagaaaaag attgcacaat tcagaaaaga gaaagagact ttcaaggaaa
     3061 aagatacata taagctattt aaaaatggaa ctctgaaaat taagcatctg aagaccgatg
     3121 atcaggatat ctacaaggta tcaatatatg atacaaaagg aaaaaatgtg ttggaaaaaa
     3181 tatttgattt gaagattcaa gagagggtct caaaaccaaa gatctcctgg acttgtatca
     3241 acacaaccct gacctgtgag gtaatgaatg gaactgaccc cgaattaaac ctgtatcaag
     3301 atgggaaaca tctaaaactt tctcagaggg tcatcacaca caagtggacc accagcctga
     3361 gtgcaaaatt caagtgcaca gcagggaaca aagtcagcaa ggaatccagt gtcgagcctg
     3421 tcagctgtcc agagaaaggt ctggacatct atctcatcat tggcatatgt ggaggaggca
     3481 gcctcttgat ggtctttgtg gcactgctcg ttttctatat caccaaaagg aaaaaacaga
     3541 ggagtcggag aaatgatgag gagctggaga caagagccca cagagtagct actgaagaaa
     3601 ggggccggaa gccccaacaa attccagctt caacccctca gaatccagca acttcccaac
     3661 atcctcctcc accacctggt catcgttccc aggcacctag tcatcgtccc ccgcctcctg
     3721 gacaccgtgt tcagcaccag cctcagaaga ggcctcctgc tccgtcgggc acacaagttc
     3781 accagcagaa aggcccgccc ctccccagac ctcgagttca gccaaaacct ccccatgggg
     3841 cagcagaaaa ctcattgtcc ccttcctcta attaaaaaag atagaaactg tctttttcaa
     3901 taaaaagcac tgtggatttc tgcctctagg gctggatgat cctccagcgc ggggatctca
     3961 tgctggagat cccgcgatta gtccaatttg ttaaagacag gatatcagtg gtccaggctc
     4021 tagttttgac tcaacaatat caccagctga agcctataga gtacgagcca tagataaaat
     4081 aaaagatttt atttagtctc cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg
     4141 gcaagctagc ttaagtaacg ccattttgca aggcatggaa aaatacataa ctgagaatag
     4201 agaagttcag atcaaggtca ggaacagatg gaacaggtcg acctagagaa ccatcagatg
     4261 tttccagggt gccccaagga cctgaaatga ccctgtgcct tatttgaact aaccaatcag
     4321 ttcgcttctc gcttctgttc gcgcgcttct gctccccgag ctcaataaaa gagcccacaa
     4381 cccctcactc ggggcgccag tcctccgatt gactgagtcg cccgggtacc cgtgtatcca
     4441 ataaaccctc ttgcagttgc atccgacttg tggtctcgct gttccttggg agggtctcct
     4501 ctgagtgatt gactacccgt cagcgggggt ctcttcacat gcagcatgta tcaaaattaa
     4561 tttggttttt ttttcttaag tatttacatt aaatggccca tagtacttaa agtaacatgg
     4621 gcttccttga aataaacatg gagtattcag aatgtgtcat aaatatttct aattttaaga
     4681 tagtatctcc attggctatc tactttttct tatatttttt tttgtcctct gtcttccatt
     4741 cgttgttgtt gttgtttgtt tgtttgtttg ttggttggtt ggttaatttt tttttaaaga
     4801 tcctacacta tagttcaagc tagactatta gctactctgt aacccagggt gacctttgaa
     4861 gtcatgggta gcctgctgtt ttagccttcc cacatctaag attacaggta tgagctatca
     4921 tttttggtat attgattgat tgattgattg atgtgtgtgt gtgtgattgt gtttgtgtgt
     4981 gtgattgtgt atatgtgtgt atggttgtgt gtgattgtgt gtatgtatgt ttgtgtgtga
     5041 ttgtgtgtgt gtgattgtgc atgtgtgtgt gtgtgattgt gtttatgtgt atgattgtgt
     5101 gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gttgtgtata tatatttatg
     5161 gtagtgagag gcaacgctcc ggctcaggtg tcaggttggt ttttgagaca gagtctttcg
     5221 cttagcttgg aattaattcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     5281 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     5341 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     5401 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     5461 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     5521 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     5581 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     5641 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     5701 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     5761 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     5821 cttcgggaag cgtggcgctt tctcaatgct cacgctgtag gtatctcagt tcggtgtagg
     5881 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     5941 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     6001 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     6061 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
     6121 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     6181 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     6241 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     6301 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     6361 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     6421 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     6481 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     6541 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     6601 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     6661 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     6721 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     6781 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     6841 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     6901 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     6961 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     7021 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     7081 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     7141 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     7201 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     7261 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     7321 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     7381 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
     7441 aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc
     7501 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
     7561 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg
     7621 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
     7681 gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg
     7741 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg
     7801 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg
     7861 ccagtgccaa gctagctttg ctcttaggag tttcctaata catcccaaac tcaaatatat
     7921 aaagcatttg acttgttcta tgccctaggg ggcgggggga agctaagcca gcttttttta
     7981 acatttaaaa tgttaattcc attttaaatg cacagatgtt tttatttcat aagggtttca
     8041 atgtgcatga atgctgcaat attcctgtta ccaaagctag tataaataaa aatagataaa
     8101 cgtggaaatt acttagagtt tctgtcatta acgtttcctt cctcagttga caacataaat
     8161 gcgctgctga gaagccagtt tgcatctgtc aggatcaatt tcccattatg ccagtcatat
     8221 taattactag tcaattagtt gatttttatt tttgacatat acatg
//
