LOCUS       sequence-101870-       17201 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Fok1:dCas9 under control of act5C promoter and SV40 3'UTR.
            For ubiquitous high specificity genome engineering in Drosophila 
            melanogaster..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17201)
  TITLE     CRISPRflydesign
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 17201)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAct-Fok1:dCas9
FEATURES             Location/Qualifiers
     source          1..17201
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     623..642
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     gene            666..4802
                     /gene="white"
                     /label=mini-white
                     /note="This modified version of the white gene lacks part 
                     of the first intron."
     primer_bind     complement(675..693)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(1145..1216,1618..1891,1966..2620,2682..2997,3218..3349,
                     3420..4034)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This modified version of the white gene lacks part 
                     of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    4809..4842
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        4990..7445
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5c promoter"
     CDS             9213..9233
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9240..9827
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQR
                     YVEENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
     CDS             join(9843..9866,9867..9869,9870..12353,12354..12359,
                     12360..12425,12426..12428,12429..13943)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=Cas9m4
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the mutations D10A, D839A, 
                     H840A, and N863A (Mali et al., 2013)"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(9843..9866,9867..9869,9870..12356,12357..12359,
                     12360..13943)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             9843..13943
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             13956..13976
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          14075..14140
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             14270..14290
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    14562..14696
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(14599..14618)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     14653..14672
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    14725..14794
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        complement(15013..15031)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(15052..15068)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(15052..15068)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(15065..15087)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    15076..15092
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(15100..15106,15107..15124,15125..15130))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    15145..15166
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(15283..15300)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(15454..16042)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(15534..15553)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(16213..17004,17005..17073))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     16836..16855
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(17074..17178)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtacgtaccg
      661 ggcccctagt atgtatgtaa gttaataaaa cccatttttg cggaaagtag ataaaaaaaa
      721 catttttttt ttttactgca ctggatatca ttgaacttat ctgatcagtt ttaaatttac
      781 ttcgatccaa gggtatttga tgtaccaggt tctttcgatt acctctcact caaaatgaca
      841 ttccactcaa agtcagcgct gtttgcctcc ttctctgtcc acagaaatat cgccgtctct
      901 ttcgccgctg cgtccgctat ctctttcgcc accgtttgta gcgttacgta gcgtcaatgt
      961 ccgccttcag ttgcattttg tcagcggttt cgtgacgaag ctccaagcgg tttacgccat
     1021 caattaaaca caaagtgctg tgccaaaact cctctcgctt cttatttttg tttgtttttt
     1081 gagtgattgg ggtggtgatt ggttttgggt gggtaagcag gggaaagtgt gaaaaatccc
     1141 ggcaatgggc caagaggatc aggagctatt aattcgcgga ggcagcaaac acccatctgc
     1201 cgagcatctg aacaatgtga gtagtacatg tgcatacatc ttaagttcac ttgatctata
     1261 ggaactgcga ttgcaacatc aaattgtctg cggcgtgaga actgcgaccc acaaaaatcc
     1321 caaaccgcaa ttgcacaaac aaatagtgac acgaaacaga ttattctggt agctgttctc
     1381 gctatataag acaatttttg agatcatatc atgatcaaga catctaaagg cattcatttt
     1441 cgactatatt cttttttaca aaaaatataa caaccagata ttttaagctg atcctagatg
     1501 cacaaaaaat aaataaaagt ataaacctac ttcgtaggat acttcggggt actttttgtt
     1561 cggggttaga tgagcataac gcttgtagtt gatatttgag atcccctatc attgcagggt
     1621 gacagcggag cggcttcgca gagctgcatt aaccagggct tcgggcaggc caaaaactac
     1681 ggcacgctcc ggccacccag tccgccggag gactccggtt cagggagcgg ccaactagcc
     1741 gagaacctca cctatgcctg gcacaatatg gacatctttg gggcggtcaa tcagccgggc
     1801 tccggatggc ggcagctggt caaccggaca cgcggactat tctgcaacga gcgacacata
     1861 ccggcgccca ggaaacattt gctcaagaac ggtgagtttc tattcgcagt cggctgatct
     1921 gtgtgaaatc ttaataaagg gtccaattac caatttgaaa ctcagtttgc ggcgtggcct
     1981 atccgggcga acttttggcc gtgatgggca gttccggtgc cggaaagacg accctgctga
     2041 atgcccttgc ctttcgatcg ccgcagggca tccaagtatc gccatccggg atgcgactgc
     2101 tcaatggcca acctgtggac gccaaggaga tgcaggccag gtgcgcctat gtccagcagg
     2161 atgacctctt tatcggctcc ctaacggcca gggaacacct gattttccag gccatggtgc
     2221 ggatgccacg acatctgacc tatcggcagc gagtggcccg cgtggatcag gtgatccagg
     2281 agctttcgct cagcaaatgt cagcacacga tcatcggtgt gcccggcagg gtgaaaggtc
     2341 tgtccggcgg agaaaggaag cgtctggcat tcgcctccga ggcactaacc gatccgccgc
     2401 ttctgatctg cgatgagccc acctccggac tggactcatt taccgcccac agcgtcgtcc
     2461 aggtgctgaa gaagctgtcg cagaagggca agaccgtcat cctgaccatt catcagccgt
     2521 cttccgagct gtttgagctc tttgacaaga tccttctgat ggccgagggc agggtagctt
     2581 tcttgggcac tcccagcgaa gccgtcgact tcttttccta gtgagttcga tgtgtttatt
     2641 aagggtatct agcattacat tacatctcaa ctcctatcca gcgtgggtgc ccagtgtcct
     2701 accaactaca atccggcgga cttttacgta caggtgttgg ccgttgtgcc cggacgggag
     2761 atcgagtccc gtgatcggat cgccaagata tgcgacaatt ttgctattag caaagtagcc
     2821 cgggatatgg agcagttgtt ggccaccaaa aatttggaga agccactgga gcagccggag
     2881 aatgggtaca cctacaaggc cacctggttc atgcagttcc gggcggtcct gtggcgatcc
     2941 tggctgtcgg tgctcaagga accactcctc gtaaaagtgc gacttattca gacaacggtg
     3001 agtggttcca gtggaaacaa atgatataac gcttacaatt cttggaaaca aattcgctag
     3061 attttagtta gaattgcctg attccacacc cttcttagtt tttttcaatg agatgtatag
     3121 tttatagttt tgcagaaaat aaataaattt catttaactc gcgaacatgt tgaagatatg
     3181 aatattaatg agatgcgagt aacattttaa tttgcagatg gttgccatct tgattggcct
     3241 catctttttg ggccaacaac tcacgcaagt gggcgtgatg aatatcaacg gagccatctt
     3301 cctcttcctg accaacatga cctttcaaaa cgtctttgcc acgataaatg taagtcttgt
     3361 ttagaataca tttgcatatt aataatttac taactttcta atgaatcgat tcgatttagg
     3421 tgttcacctc agagctgcca gtttttatga gggaggcccg aagtcgactt tatcgctgtg
     3481 acacatactt tctgggcaaa acgattgccg aattaccgct ttttctcaca gtgccactgg
     3541 tcttcacggc gattgcctat ccgatgatcg gactgcgggc cggagtgctg cacttcttca
     3601 actgcctggc gctggtcact ctggtggcca atgtgtcaac gtccttcgga tatctaatat
     3661 cctgcgccag ctcctcgacc tcgatggcgc tgtctgtggg tccgccggtt atcataccat
     3721 tcctgctctt tggcggcttc ttcttgaact cgggctcggt gccagtatac ctcaaatggt
     3781 tgtcgtacct ctcatggttc cgttacgcca acgagggtct gctgattaac caatgggcgg
     3841 acgtggagcc gggcgaaatt agctgcacat cgtcgaacac cacgtgcccc agttcgggca
     3901 aggtcatcct ggagacgctt aacttctccg ccgccgatct gccgctggac tacgtgggtc
     3961 tggccattct catcgtgagc ttccgggtgc tcgcatatct ggctctaaga cttcgggccc
     4021 gacgcaagga gtagccgaca tatatccgaa ataactgctt gttttttttt tttaccatta
     4081 ttaccatcgt gtttactgtt tattgccccc tcaaaaagct aatgtaatta tatttgtgcc
     4141 aataaaaaca agatatgacc tatagaatac aagtatttcc ccttcgaaca tccccacaag
     4201 tagactttgg atttgtcttc taaccaaaag acttacacac ctgcatacct tacatcaaaa
     4261 actcgtttat cgctacataa aacaccggga tatatttttt atatacatac ttttcaaatc
     4321 gcgcgccctc ttcataattc acctccacca caccacgttt cgtagttgct ctttcgctgt
     4381 ctcccacccg ctctccgcaa cacattcacc ttttgttcga cgaccttgga gcgactgtcg
     4441 ttagttccgc gcgattcggt tcgctcaaat ggttccgagt ggttcatttc gtctcaatag
     4501 aaattagtaa taaatatttg tatgtacaat ttatttgctc caatatattt gtatatattt
     4561 ccctcacagc tatatttatt ctaatttaat attatgactt tttaaggtaa ttttttgtga
     4621 cctgttcgga gtgattagcg ttacaatttg aactgaaagt gacatccagt gtttgttcct
     4681 tgtgtagatg catctcaaaa aaatggtggg cataatagtg ttgtttatat atatcaaaaa
     4741 taacaactat aataataaga atacatttaa tttagaaaat gcttggattt cactggaact
     4801 aggctagcat aacttcgtat aatgtatgct atacgaagtt atgctagcgg atccaagctt
     4861 tgcggccgca attctatatt ctaaaaacac aaatgatact tctaaaaaaa aatcatgaat
     4921 ggcatcaact ctgaatcaaa tctttgcaga tgcacctact tctcatttcc actgtcacat
     4981 catttttcca gatctcgctg cctgttatgt ggcccacaaa ccaagacacg ttttatggcc
     5041 attaaagctg gctgatcgtc gccaaacacc aaatacataa tgaatatgta cacattcgag
     5101 aaagaagcga tcaaagaagc gtcttcgggc ggagtaggag aatgcggagg agaaggagaa
     5161 cgagctgatc tagtatctct ccacaatcca atgccaactg accaactggc catattcgga
     5221 gcaatttgaa gccaatttcc atcgcctggc gatcgctcca ttcttggcta tatgtttttc
     5281 accgttaccc ggggccattt tcaaagactc gtcggcaaga taagattgtg tcactcgctg
     5341 tctctcttca tttgtcgaag aatgctgagg aatttcgcga tgacgtcggc gagtattttg
     5401 aagaatgaga ataatttgta tttatacgaa aatcagttag tggaattttc tacaaaaaca
     5461 tgttatctat agataatttt gttgcaaaat atgttgacta tgacaaagat tgtatgtata
     5521 tacctttaat gtattctcat tttcttatgt atttataatg gcaatgatga tactgatgat
     5581 attttaagat gatgccagac cacaggctga tttctgcgtc ttttgccgaa cgcagtgcat
     5641 gtgcggttgt tgttttttgg aatagtttca attttcggac tgtccgcttt gatttcagtt
     5701 tcttggctta ttcaaaaagc aaagtaaagc caaaaaagcg agatggcaat accaaatgcg
     5761 gcaaaacggt agtggaagga aaggggtgcg gggcagcgga aggaagggtg gggcggggcg
     5821 tggcggggtc tgtggctggg cgcgacgtca ccgacgttgg agccactcct ttgaccatgt
     5881 gtgcgtgtgt gtattattcg tgtctcgcca ctcgccggtt gtttttttct ttttatctcg
     5941 ctctctctag cgccatctcg tacgcatgct caacgcaccg catgttgccg tgtcctttat
     6001 gcgtcatttt ggctcgaaat aggcaattat ttaaacaaag attagtcaac gaaaacgcta
     6061 aaataaataa gtctacaata tggttactta ttgccatgtg tgtgcagcca acgatagcaa
     6121 caaaagcaac aacacagtgg ctttccctct ttcacttttt gtttgcaagc gcgtgcgagc
     6181 aagacggcac gaccggcaaa cgcaattacg ctgacaaaga gcagacgaag ttttggccga
     6241 aaaacatcaa ggcgcctgat acgaatgcat ttgcaataac aattgcgata tttaatattg
     6301 tttatgaagc tgtttgactt caaaacacac aaaaaaaaaa ataaaacaaa ttatttgaaa
     6361 gagaattagg aatcggacag cttatcgtta cgggctaaca gcacaccgag acgaaatagc
     6421 ttacctgacg tcacagcctc tggaagaact gccgccaagc agacgatgca gaggacgaca
     6481 catagagtag cggagtaggc cagcgtagta cgcatgtgct tgtgtgtgag gcgtctctct
     6541 cttcgtctcc tgtttgcgca aacgcataga ctgcactgag aaaatcgatt acctattttt
     6601 tatgaatgaa tatttgcact attactattc aaaactatta agatagcaat cacattcaat
     6661 agccaaatac tataccacct gagcgatgca acgaaatgat caatttgagc aaaaatgctg
     6721 catatttagg acggcatcat tatagaaatg cttcttgctg tgtacttttc tctcgtctgg
     6781 cagctgtttc gccgttattg ttaaaaccgg cttaagttag gtgtgttttc tacgactagt
     6841 gatgccccta ctagaagatg tgtgttgcac aaatgtccct gaataaccaa tttgaagtgc
     6901 agatagcagt aaacgtaagc taatatgaat attatttaac tgtaatgttt taatatcgct
     6961 ggacattact aataaaccca ctataaacac atgtacatat gtatgttttg gcatacaatg
     7021 agtagttggg gaaaaaatgt gtaaaagcac cgtgaccatc acagcataaa gataaccagc
     7081 tgaagtatcg aatatgagta acccccaaat tgaatcacat gccgcaactg ataggaccca
     7141 tggaagtaca ctcttcatgg cgatatacaa gacacacaca agcacgaaca cccagttgcg
     7201 gaggaaattc tccgtaaatg aaaacccaat cggcgaacaa ttcataccca tatatggtaa
     7261 aagttttgaa cgcgacttga gagcggagag cattgcggct gataaggttt tagcgctaag
     7321 cgggctttat aaaacgggct gcgggaccag ttttcatatc actaccgttt gagttcttgt
     7381 gctgtgtgga tactcctccc gacacaaagc cgctccatca gccagcagtc gtctaatcca
     7441 gagacaccaa accgaaagac ttaatttata tttatttaat taattttaat aaaacacacc
     7501 aaatgtaagt agctttcccc ttcccaacaa caaaacacca tcgaaccact cccaccaaga
     7561 aaaagcaata atcgagaaaa gccgcggaaa atgtgtgatt ttttttgtaa acaaaacatt
     7621 tttttatgtg ccagtgctga aagtgatcaa aaaatactag ccacgagcta aagagttatt
     7681 gtattgacca aaactccaaa aatacccaag tttggcccta aattgtcaat caaaatacca
     7741 ataggtcgaa agacatcaaa attaacaaaa ccagggtttc aaataccata actcaagaat
     7801 caggattaca actgcagatt tcaggatata tacatacaaa ttatacgaaa ttataaaaac
     7861 caaagcaatt caatagcccc aactcaaatg ttaggatcta atatagtgtt taaagccaag
     7921 ctcgctgatg tgggcgtgtc acgatttcac ccaaagatat gccaaattac gaattgcaaa
     7981 tcaattcgcc aacactttct ttttttccca cgccctaaaa caccagatca tccataaatg
     8041 tacatacata cagtatatgc atattataat ctgtaaaact agatcaggtt cttgaaaata
     8101 gtgacgtagg cagccgtttt ggctgaagca gaaatttttg ccggtttttc aaagttgtag
     8161 ttgcaaaaat ggagaaaacc ttcgagcatt cgttcatata cacacactca cgcgcaaaat
     8221 aacgagagag agtgtatgtg tgtgtgagag agcgaaagcc agacgacggt ttgcttttcg
     8281 cctcgaaaca tgaccatata tggtcacaaa acttggccgc cgcaattcaa cacaccagcg
     8341 ctctccttcg cacccatagc gaccatggcg cggagcgagc gagatggcga gagcgagcga
     8401 cgcctatggc gacgtcgacg caggcagcga ttgaaaaacg cagttaactg gcattcaaca
     8461 ttcaccagcc actttcagtc ggtttattcc agtcattcct ttcaaaccgt gcggtcgctt
     8521 agctcagcct cgccacttgc gtttacagta gttttcacgc cttgaatttg ttaaatcgaa
     8581 caaaaaggta aagtttaact agctttgaaa agtttcgtgg ctcttaattg ttaaattttc
     8641 tagagtgcgt ttagtgtttt tttttttttt tattttgtaa tgttaatttc gggttccaat
     8701 tcgagtttta ggcagccgcc attttaaggg cgcatacaca caggcaactg tgctctcttt
     8761 gcggctttct tttgcaccgg cattcgttaa gtgtcgtcta gaagcttctc ccctcccttt
     8821 tcggcatatt cgtattgtgg ttttaatttt tcggggcggg gccttctatt ttgtaactgt
     8881 tcttttaatt tcttattaca attcgatcgc aagtgaaaat cagttttcaa tcggaaaagt
     8941 atttttttat gaaatttttt ttgtccaaga ttaaaatttt gtactaaaaa aacgtacatt
     9001 gcattgagtg atttttaatt gtacacgaaa aacaagttag tttgttatga caattgtact
     9061 ttggtagacc agcgcagtcc aaggaaacca cgcaaattct cagttttttt tttgccattt
     9121 ctacattacc aaataaggta accaaaaact aatgggaaat ccgcattctt tccattgcag
     9181 cttacaggat cgatccccgg gaattcacca tgcctaagaa gaagcggaag gtgagcagcc
     9241 aacttgtgaa gtctgaactc gaggagaaaa aatcagagtt gagacacaag ttgaagtacg
     9301 tgccacacga atacatcgag cttatcgaga tcgccagaaa cagtacccag gataggatcc
     9361 ttgagatgaa agtcatggag ttctttatga aggtctacgg ttatagagga aagcaccttg
     9421 gcggtagcag aaagcccgat ggcgccatct atactgtcgg atctcctatc gattatgggg
     9481 tgatcgtgga taccaaagct tactcaggcg ggtacaactt gcccatagga caagccgacg
     9541 agatgcagcg gtatgtcgaa gagaaccaga cgcgcaacaa gcacatcaac cccaatgaat
     9601 ggtggaaagt gtacccaagt agtgtgactg agttcaagtt cctgtttgtc tccggccact
     9661 ttaagggcaa ttataaagct cagctcacta gactcaatca catcacaaac tgcaacggag
     9721 ctgtgttgtc agtggaggag ctcctgattg gaggcgagat gatcaaagcc ggcaccctta
     9781 cactggagga ggtgcggcgg aagttcaaca atggagagat caacttcggt ggcggtggat
     9841 ccgacaagaa gtactccatt gggctcgcta tcggcacaaa cagcgtcggc tgggccgtca
     9901 ttacggacga gtacaaggtg ccgagcaaaa aattcaaagt tctgggcaat accgatcgcc
     9961 acagcataaa gaagaacctc attggcgccc tcctgttcga ctccggggag acggccgaag
    10021 ccacgcggct caaaagaaca gcacggcgca gatatacccg cagaaagaat cggatctgct
    10081 acctgcagga gatctttagt aatgagatgg ctaaggtgga tgactctttc ttccataggc
    10141 tggaggagtc ctttttggtg gaggaggata aaaagcacga gcgccaccca atctttggca
    10201 atatcgtgga cgaggtggcg taccatgaaa agtacccaac catatatcat ctgaggaaga
    10261 agcttgtaga cagtactgat aaggctgact tgcggttgat ctatctcgcg ctggcgcata
    10321 tgatcaaatt tcggggacac ttcctcatcg agggggacct gaacccagac aacagcgatg
    10381 tcgacaaact ctttatccaa ctggttcaga cttacaatca gcttttcgaa gagaacccga
    10441 tcaacgcatc cggagttgac gccaaagcaa tcctgagcgc taggctgtcc aaatcccggc
    10501 ggctcgaaaa cctcatcgca cagctccctg gggagaagaa gaacggcctg tttggtaatc
    10561 ttatcgccct gtcactcggg ctgaccccca actttaaatc taacttcgac ctggccgaag
    10621 atgccaagct tcaactgagc aaagacacct acgatgatga tctcgacaat ctgctggccc
    10681 agatcggcga ccagtacgca gacctttttt tggcggcaaa gaacctgtca gacgccattc
    10741 tgctgagtga tattctgcga gtgaacacgg agatcaccaa agctccgctg agcgctagta
    10801 tgatcaagcg ctatgatgag caccaccaag acttgacttt gctgaaggcc cttgtcagac
    10861 agcaactgcc tgagaagtac aaggaaattt tcttcgatca gtctaaaaat ggctacgccg
    10921 gatacattga cggcggagca agccaggagg aattttacaa atttattaag cccatcttgg
    10981 aaaaaatgga cggcaccgag gagctgctgg taaagcttaa cagagaagat ctgttgcgca
    11041 aacagcgcac tttcgacaat ggaagcatcc cccaccagat tcacctgggc gaactgcacg
    11101 ctatcctcag gcggcaagag gatttctacc cctttttgaa agataacagg gaaaagattg
    11161 agaaaatcct cacatttcgg ataccctact atgtaggccc cctcgcccgg ggaaattcca
    11221 gattcgcgtg gatgactcgc aaatcagaag agaccatcac tccctggaac ttcgaggaag
    11281 tcgtggataa gggggcctct gcccagtcct tcatcgaaag gatgactaac tttgataaaa
    11341 atctgcctaa cgaaaaggtg cttcctaaac actctctgct gtacgagtac ttcacagttt
    11401 ataacgagct caccaaggtc aaatacgtca cagaagggat gagaaagcca gcattcctgt
    11461 ctggagagca gaagaaagct atcgtggacc tcctcttcaa gacgaaccgg aaagttaccg
    11521 tgaaacagct caaagaagac tatttcaaaa agattgaatg tttcgactct gttgaaatca
    11581 gcggagtgga ggatcgcttc aacgcatccc tgggaacgta tcacgatctc ctgaaaatca
    11641 ttaaagacaa ggacttcctg gacaatgagg agaacgagga cattcttgag gacattgtcc
    11701 tcacccttac gttgtttgaa gatagggaga tgattgaaga acgcttgaaa acttacgctc
    11761 atctcttcga cgacaaagtc atgaaacagc tcaagaggcg ccgatataca ggatgggggc
    11821 ggctgtcaag aaaactgatc aatgggatcc gagacaagca gagtggaaag acaatcctgg
    11881 attttcttaa gtccgatgga tttgccaacc ggaacttcat gcagttgatc catgatgact
    11941 ctctcacctt taaggaggac atccagaaag cacaagtttc tggccagggg gacagtcttc
    12001 acgagcacat cgctaatctt gcaggtagcc cagctatcaa aaagggaata ctgcagaccg
    12061 ttaaggtcgt ggatgaactc gtcaaagtaa tgggaaggca taagcccgag aatatcgtta
    12121 tcgagatggc ccgagagaac caaactaccc agaagggaca gaagaacagt agggaaagga
    12181 tgaagaggat tgaagagggt ataaaagaac tggggtccca aatccttaag gaacacccag
    12241 ttgaaaacac ccagcttcag aatgagaagc tctacctgta ctacctgcag aacggcaggg
    12301 acatgtacgt ggatcaggaa ctggacatca atcggctctc cgactacgac gtggatgcca
    12361 tcgtgcccca gtcttttctc aaagatgatt ctattgataa taaagtgttg acaagatccg
    12421 ataaaaatag agggaagagt gataacgtcc cctcagaaga agttgtcaag aaaatgaaaa
    12481 attattggcg gcagctgctg aacgccaaac tgatcacaca acggaagttc gataatctga
    12541 ctaaggctga acgaggtggc ctgtctgagt tggataaagc cggcttcatc aaaaggcagc
    12601 ttgttgagac acgccagatc accaagcacg tggcccaaat tctcgattca cgcatgaaca
    12661 ccaagtacga tgaaaatgac aaactgattc gagaggtgaa agttattact ctgaagtcta
    12721 agctggtctc agatttcaga aaggactttc agttttataa ggtgagagag atcaacaatt
    12781 accaccatgc gcatgatgcc tacctgaatg cagtggtagg cactgcactt atcaaaaaat
    12841 atcccaagct tgaatctgaa tttgtttacg gagactataa agtgtacgat gttaggaaaa
    12901 tgatcgcaaa gtctgagcag gaaataggca aggccaccgc taagtacttc ttttacagca
    12961 atattatgaa ttttttcaag accgagatta cactggccaa tggagagatt cggaagcgac
    13021 cacttatcga aacaaacgga gaaacaggag aaatcgtgtg ggacaagggt agggatttcg
    13081 cgacagtccg gaaggtcctg tccatgccgc aggtgaacat cgttaaaaag accgaagtac
    13141 agaccggagg cttctccaag gaaagtatcc tcccgaaaag gaacagcgac aagctgatcg
    13201 cacgcaaaaa agattgggac cccaagaaat acggcggatt cgattctcct acagtcgctt
    13261 acagtgtact ggttgtggcc aaagtggaga aagggaagtc taaaaaactc aaaagcgtca
    13321 aggaactgct gggcatcaca atcatggagc gatcaagctt cgaaaaaaac cccatcgact
    13381 ttctcgaggc gaaaggatat aaagaggtca aaaaagacct catcattaag cttcccaagt
    13441 actctctctt tgagcttgaa aacggccgga aacgaatgct cgctagtgcg ggcgagctgc
    13501 agaaaggtaa cgagctggca ctgccctcta aatacgttaa tttcttgtat ctggccagcc
    13561 actatgaaaa gctcaaaggg tctcccgaag ataatgagca gaagcagctg ttcgtggaac
    13621 aacacaaaca ctaccttgat gagatcatcg agcaaataag cgaattctcc aaaagagtga
    13681 tcctcgccga cgctaacctc gataaggtgc tttctgctta caataagcac agggataagc
    13741 ccatcaggga gcaggcagaa aacattatcc acttgtttac tctgaccaac ttgggcgcgc
    13801 ctgcagcctt caagtacttc gacaccacca tagacagaaa gcggtacacc tctacaaagg
    13861 aggtcctgga cgccacactg attcatcagt caattacggg gctctatgaa acaagaatcg
    13921 acctctctca gctcggtgga gacagcaggg ctgaccccaa gaagaagagg aaggtgtgac
    13981 tcgagggtac ctctagagga tctttgtgaa ggaaccttac ttctgtggtg tgacataatt
    14041 ggacaaacta cctacagaga tttaaagctc taaggtaaat ataaaatttt taagtgtata
    14101 atgtgttaaa ctactgattc taattgtttg tgtattttag attccaacct atggaactga
    14161 tgaatgggag cagtggtgga atgcctttaa tgaggaaaac ctgttttgct cagaagaaat
    14221 gccatctagt gatgatgagg ctactgctga ctctcaacat tctactcctc caaaaaagaa
    14281 gagaaaggta gaagacccca aggactttcc ttcagaattg ctaagttttt tgagtcatgc
    14341 tgtgtttagt aatagaactc ttgcttgctt tgctatttac accacaaagg aaaaagctgc
    14401 actgctatac aagaaaatta tggaaaaata tttgatgtat agtgccttga ctagagatca
    14461 taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc
    14521 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt
    14581 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
    14641 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatccact
    14701 agtgtcgacg atgtaggtca cggtctcgaa gccgcggtgc gggtgccagg gcgtgccctt
    14761 gggctccccg ggcgcgtact ccacctcacc catctggtcc atcatgatga acgggtcgag
    14821 gtggcggtag ttgatcccgg cgaacgcgcg gcgcaccggg aagccctcgc cctcgaaacc
    14881 gctgggcgcg gtggtcacgg tgagcacggg acgtgcgacg gcgtcggcgg gtgcggatac
    14941 gcggggcagc gtcagcgggt tctcgacggt cacggcgggc atgtcgacac tagttctagc
    15001 cagcttttgt tccctttagt gagggttaat ttcgagcttg gcgtaatcat ggtcatagct
    15061 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
    15121 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
    15181 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
    15241 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
    15301 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
    15361 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
    15421 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
    15481 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
    15541 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
    15601 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
    15661 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
    15721 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
    15781 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
    15841 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
    15901 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
    15961 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
    16021 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
    16081 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
    16141 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
    16201 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
    16261 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
    16321 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
    16381 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
    16441 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
    16501 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
    16561 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
    16621 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
    16681 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
    16741 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
    16801 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
    16861 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
    16921 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
    16981 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
    17041 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
    17101 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
    17161 acaaataggg gttccgcgca catttccccg aaaagtgcca c
//
