LOCUS       sequence-101889-         776 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human OTUD5 (OTU domain) in E. coli..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Mevissen TE, Hospenthal MK, Geurink PP, Elliott PR, Akutsu M, 
            Arnaudo N, Ekkebus R, Kulathu Y, Wauer T, El Oualid F, Freund SM, 
            Ovaa H, Komander D
  TITLE     OTU Deubiquitinases Reveal Mechanisms of Linkage Specificity and 
            Enable Ubiquitin Chain Restriction Analysis.
  JOURNAL   Cell. 2013 Jul 3;154(1):169-84. doi: 10.1016/j.cell.2013.05.046.
  PUBMED    23827681
REFERENCE   2  (bases 1 to 776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.05"; journalName: "Cell"; date: "2013-07-3- 3";
            volume: "154"; issue: "1"; pages: "169-84"
COMMENT     Sequence Label: pOPINK-OTUD5 (OTU, aa 171-358)
FEATURES             Location/Qualifiers
     source          1..776
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     69..91
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             130..153
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             741..758
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cgtattgaag ctatcccaca aattgataag tacttgaaat ccagcaagta tatagcatgg
       61 cctttgcagg gctggcaagc cacgtttggt ggtggcgacc atcctccaaa atcggatctg
      121 agcagcggtc tggaagttct gtttcagggc ccggtcggcg caggctacaa cagtgaggac
      181 gagtatgagg cggctgcagc acgcatcgag gctatggacc ctgccactgt cgagcagcag
      241 gagcattggt ttgaaaaggc cctacgagac aagaagggct tcatcatcaa gcagatgaag
      301 gaggatggcg cctgtctctt ccgggctgta gctgaccagg tgtatggaga ccaggacatg
      361 catgaggttg tgcgaaagca ttgcatggac tatctgatga agaatgccga ctacttctcc
      421 aactatgtca cagaggactt taccacctac attaacagga agcggaaaaa caattgccat
      481 ggcaaccaca ttgagatgca ggccatggca gagatgtaca accgtcctgt ggaggtgtac
      541 cagtacagca caggtacttc tgcagtggaa cccatcaaca cattccatgg gatacatcaa
      601 aacgaggacg aacccattcg tgttagctac catcggaata tccactataa ttcagtggtg
      661 aatcctaaca aggccaccat tggtgtgggg ctgggcctgc catcattcaa accagggtaa
      721 agctttctag accatttaaa caccaccacc accaccactg agatccggct gctaac
//
