LOCUS       sequence-101913-         947 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standardized mini-Tn5 delivery plasmid, streptomycin/spectinomycin 
            resistant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Martinez-Garcia E, Aparicio T, de Lorenzo V, Nikel PI
  TITLE     New transposon tools tailored for metabolic engineering of 
            gram-negative microbial cell factories.
  JOURNAL   Front Bioeng Biotechnol. 2014 Oct 28;2:46. doi: 
            10.3389/fbioe.2014.00046. eCollection 2014.
  PUBMED    25389526
REFERENCE   2  (bases 1 to 947)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Bioeng Biotechnol."; date: "2014-10-28"; volume: "2:46. doi"; pages:
            " 10.3389/fbioe.2014.00046. eCollection 2014"
COMMENT     Sequence Label: pBAMD1-4
FEATURES             Location/Qualifiers
     source          1..947
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    39..57
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase 
                     recognition (Reznikoff et al., 2004)"
     CDS             complement(150..941)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 gtcattactg gatctatcaa caggagtcca agactagtct gtctcttata cacatcttca
       61 gagattttat gcttgtaaac cgttttgtga aaaaattttt aaaataaaaa aggggacctc
      121 tagggtccgc aattaattag acaattgtct tatttgccga ctaccttggt gatctcgcct
      181 ttcacgtagt ggacaaattc ttccaactga tctgcgcgcg aggccaagcg atcttcttct
      241 tgtccaagat aagcctgtct agcttcaagt atgacgggct gatactgggc cggcaggcgc
      301 tccattgccc agtcggcagc gacatccttc ggcgcgattt tgccggttac tgcgctgtac
      361 caaatgcggg acaacgtaag cactacattt cgctcatcgc cagcccagtc gggcggcgag
      421 ttccatagcg ttaaggtttc atttagcgcc tcaaatagat cctgttcagg aaccggatca
      481 aagagttcct ccgccgctgg acctaccaag gcaacgctat gttctcttgc ttttgtcagc
      541 aagatagcca gatcaatgtc gatcgtggct ggctcgaaga tacctgcaag aatgtcattg
      601 cgctgccatt ctccaaattg cagttcgcgc ttagctggat aacgccacgg aatgatgtcg
      661 tcgtgcacaa caatggtgac ttctacagcg cggagaatct cgctctctcc aggggaagcc
      721 gaagtttcca aaaggtcgtt gatcaaagct cgccgcgttg tttcatcaag ccttacggtc
      781 accgtaacca gcaaatcaat atcactgtgt ggcttcaggc cgccatccac tgcggagccg
      841 tacaaatgta cggccagcaa cgtcggttcg agatggcgct cgatgacgcc aactacctct
      901 gatagttgag tcgatacttc ggcgatcacc gcttccctca tgatgtt
//
