LOCUS       sequence-101948-         918 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a fusion protein of FKBP12(F36V)-linker peptide 2- 
            Cre(19-59).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 918)
  TITLE     Herman's lab DiCre2-59.F2 plasmid
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pcDNA_DiCre2_59.F2
FEATURES             Location/Qualifiers
     source          1..918
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     68..85
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(285..605)
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=FKBP-V
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             complement(612..632)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      635..644
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(711..730)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(712..730)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(775..874)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(804..824)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 gtgggagtgg caccttccag ggtcaaggaa ggcacggggg aggggcaaac aacagatggc
       61 tggcaactag aaggcacagt cgaggctgat cagcgggttt aaacgggccc tctagactcg
      121 agtcagttca gcttgcacca ggcagcccag gatctgcaca cagacaggag catcttccag
      181 gtgtgttcag agaaggcctg cctgtccctg aacatgtcca tcaggttctt cctgacttca
      241 tcagaggtgg atccgttgct ggcgccgggg ttgctggggc tagcttccag ttttagaagc
      301 tccacatcga agacgagagt ggcatgtggt gggatgatgc ctgggtgccc agtggcacca
      361 taggcataat ctggagatat agtcagtttg gctctctgac ccacactcat ctgggcaacc
      421 ccttcttccc agcctcggat cacctcctgc ttgcctagca taaacttaaa gggcttgttt
      481 ctgtcccggg aggaatcaac tttctttcca tcttcaagca tcccggtgta gtgcaccacg
      541 caggtctggc cgcgcttggg gaaggtgcgc ccgtctcctg gggagatggt ttccacctgc
      601 actcctctag acacctttct cttcttctta ggggccatgg tggcgatccc ccgggctgca
      661 ggaattccac cacactggac tagtggatcg atcctagcca gcttgggtct ccctatagtg
      721 agtcgtatta atttcgataa gccagtaagc agtgggttct ctagttagcc agagagctct
      781 gcttatatag acctcccacc gtacacgcct accgcccatt tgcgtcaatg gggcggagtt
      841 gttacgacat tttggaaagt cccgttgatt ttggtgccaa aacaaactcc cattgacgtc
      901 aatggggtgg agacttgg
//
