LOCUS       sequence-10197-e        2756 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2756)
  TITLE     shMDC1 plasmids
REFERENCE   2  (bases 1 to 2756)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p16 in pENTR1A (w97-1)
FEATURES             Location/Qualifiers
     source          1..2756
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      103..189
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      281..308
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     316..339
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     protein_bind    358..457
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(985..1084)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1196..1215)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1207..2016
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVSLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1274..1293)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2106..2694
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2595..2614
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagca tggatctcgg
       61 ggacgtctaa ctactaagcg agagtaggga actgccaggc atcaaataaa acgaaaggct
      121 cagtcggaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
      181 aggacaaatc cgccgggagc ggatttgaac gttgtgaagc aacggcccgg agggtggcgg
      241 gcaggacgcc cgccataaac tgccaggcat caaactaagc agaaggccat cctgacggat
      301 ggcctttttg cgtttctaca aactcttcct gttagttagt tacttaagct cgggccccaa
      361 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc
      421 ttttttataa tgccaacttt gtacaaaaaa gcaggcttta aaggaaccaa ttcagtcgac
      481 tggatccagc atggagcctt cggctgactg gctggccacg gccgcggccc ggggtcgggt
      541 agaggaggtg cgggcgctgc tggaggcggg ggcgctgccc aacgcaccga atagttacgg
      601 tcggaggccg atccaggtca tgatgatggg cagcgcccga gtggcggagc tgctgctgct
      661 ccacggcgcg gagcccaact gcgccgaccc cgccactctc acccgacccg tgcacgacgc
      721 tgcccgggag ggcttcctgg acacgctggt ggtgctgcac cgggccgggg cgcggctgga
      781 cgtgcgcgat gcctggggcc gtctgcccgt ggacctggct gaggagctgg gccatcgcga
      841 tgtcgcacgg tacctgcgcg cggctgcggg gggcaccaga ggcagtaacc atgcccgcat
      901 agatgccgcg gaaggtccct cagacatccc cgattgaaag gaattctgca gatatccatc
      961 acactggccg ctcgagatat ctagacccag ctttcttgta caaagttggc attataagaa
     1021 agcattgctt atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta
     1081 tttgccatcc agctgcagct ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa
     1141 gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag taatacaagg
     1201 ggtgttatga gccatattca acgggaaacg tcgaggccgc gattaaattc caacatggat
     1261 gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc
     1321 tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc
     1381 gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct
     1441 cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg
     1501 atccccggaa aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt
     1561 gttgatgcgc tggcagtgtc cctgcgccgg ttgcattcga ttcctgtttg taattgtcct
     1621 tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg
     1681 gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa
     1741 gaaatgcata aacttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca
     1801 cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc
     1861 ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct
     1921 ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa
     1981 ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa ttggttgtaa
     2041 cattattcag attgggcccc gttccactga gcgtcagacc cggtagaaaa gatcaaagga
     2101 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     2161 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     2221 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     2281 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     2341 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     2401 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     2461 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     2521 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     2581 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     2641 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     2701 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgtt
//
