LOCUS       sequence-102016-         935 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ER targeted transmembrane domain with monomeric EGFP oriented 
            towards the cytoplasm. Used for assessing fluorescent protein 
            oligomerization..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Costantini LM, Fossati M, Francolini M, Snapp EL
  TITLE     Assessing the tendency of fluorescent proteins to oligomerize under 
            physiologic conditions.
  JOURNAL   Traffic. 2012 May;13(5):643-9. doi: 
            10.1111/j.1600-0854.2012.01336.x. Epub 2012 Feb 20.
  PUBMED    22289035
REFERENCE   2  (bases 1 to 935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/j.1600-0854.2012.01336.x"; journalName: "Traffic"; date: 
            "2012-05"; volume: "13"; issue: "5"; pages: "643-9"
COMMENT     Sequence Label: CyTERM-mGFP
FEATURES             Location/Qualifiers
     source          1..935
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      201..210
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(207..209,210..212,213..926)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLK
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(252..273)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(513..532)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 cgctagcgct accggactca gatctggtac cccttgcagc ttccagttgt caggaagaga
       61 aggctcccat ggatcctgtg gtggtgctgg ggctctgtct ctcctgtttg cttctccttt
      121 cactctggaa acagagctat gggggaggga agcttcgaat tctgcagtcg acggtaccgc
      181 gggcccggga tccaccggtc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg
      241 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
      301 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
      361 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct
      421 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
      481 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
      541 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
      601 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
      661 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca
      721 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
      781 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc
      841 ccaacgagaa gcgcgatcac atggtcctta aggagttcgt gaccgccgcc gggatcactc
      901 tcggcatgga cgagctgtac aagtaaagcg gccgc
//
