LOCUS       sequence-102040-         779 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  const: miRFKBP5_GFP_IRES_Puro; ind: mC-FRB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Ballister ER, Riegman M, Lampson MA
  TITLE     Recruitment of Mad1 to metaphase kinetochores is sufficient to 
            reactivate the mitotic checkpoint.
  JOURNAL   J Cell Biol. 2014 Mar 17;204(6):901-8. doi: 10.1083/jcb.201311113.
  PUBMED    24637323
REFERENCE   2  (bases 1 to 779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2014-03-17- 17"; volume: "204"; 
            issue: "6"; pages: "901-8"
COMMENT     Sequence Label: pERB122
FEATURES             Location/Qualifiers
     source          1..779
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      6..15
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(77..96)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     ncRNA           complement(171..212)
                     /label=3' miR-155
                     /note="sequence downstream of the precursor of mouse 
                     miR-155 microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     ncRNA           complement(276..302)
                     /label=5' miR-155
                     /note="sequence upstream of the precursor of mouse miR-155 
                     microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     protein_bind    complement(458..491)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     polyA_signal    703..751
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 gctcaccatg gtggcgaccg gctcgaggcg gccgcctgca gggcgcgccg gtggatcccg
       61 ggcccgcggt accgacacct gtggagagaa aggcaaagtg gatgtcagta agaccaatag
      121 gtgcctatct ggtccacgcg tatgcgatat cagcagctag cgccgaattc ggccatttgt
      181 tccatgtgag tgctagtaac aggccttgtg tcctgatatg gattcatgca catggtcagt
      241 cagtggccaa aaccatgtgc acatgaatcc atatcagcat acagccttca gcaagcctcc
      301 agagctctag aagtcgaccg aaacgcaaga gtcttctctg tctcgacaag cccagtttct
      361 attggtctcc ttaaacctgt cttgtaacct tgatacttac ctgcccagtg cctcacgacc
      421 aacttctgca aggctaccgg atacgtactg cagggtgata acttcgtata ggatacttta
      481 tacgaagtta tttattctcg aatgggggac cccgtcccta acccacgggg ccagtggcta
      541 tggcagggcc tgccgccccg acgttggctg cgagccctgg gccttcaccc gaacttgggg
      601 ggtggggtgg ggaaaaggaa gaaacgcggg cgtattggcc ccaatggggt ctcggtgggg
      661 tatcgacaga gtgccagccc tgggaccgaa ccccgcgttt atgaacaaac gacccaacac
      721 ccgtgcgttt tattctgtct ttttattgcc gtcatagcgc gggttccttc cggtattgt
//
