LOCUS       sequence-102126-        1048 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mCherry fused to LINuS, a light-inducible nuclear localization 
            signal (biNLS11/PKIt NES).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Niopek D, Benzinger D, Roensch J, Draebing T, Wehler P, Eils R, Di 
            Ventura B
  TITLE     Engineering light-inducible nuclear localization signals for precise
            spatiotemporal control of protein dynamics in living cells.
  JOURNAL   Nat Commun. 2014 Jul 14;5:4404. doi: 10.1038/ncomms5404.
  PUBMED    25019686
REFERENCE   2  (bases 1 to 1048)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-07-14"; volume: "5:4404. doi"; pages: " 
            10.1038/ncomms5404"
COMMENT     Sequence Label: pDN43
FEATURES             Location/Qualifiers
     source          1..1048
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     69..86
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     primer_bind     complement(972..991)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 agtgggagtg gcaccttcca gggtcaagga aggcacgggg gaggggcaaa caacagatgg
       61 ctggcaacta gaaggcacag tcgaggctga tcagcggttt aaacttaagc ttggtaccga
      121 gctcggatcc actagtccag tgtggtggaa ttctgcagat atccagcaca gtggcggccg
      181 ctcgagctag tccagtttct ttctcttggt cttggctgcg gctgccaggt tggcgtcggg
      241 cagtctcttt gcggcctcgt caatattttc tgcagttttc ttaatcagca tgactccctc
      301 tctctcggca gcatctcgga catgctcagt tccatccaac tgaaccccaa taaagtactg
      361 gacatctccc ttctgatctc gcataggctg caagtgaaag aggttccaga actttttacc
      421 actctttgta taattaatca gctgaacagt gacctctgtt tggttatcta tggcatctct
      481 aatttttctc actgtcgcgc gatcagtttc aggaccttgt agaaacctgc agtttcttcc
      541 caaaatttct tcacggctat attctgtcaa ctgcaagaaa ctatcggacg cgaatataat
      601 gggattatct ggcaatcttg ggtcagtaat gacaaagttc ttctcaatac gttcaagtgt
      661 agtagccaaa cttccacctg aacctccaga tccacccttg tacagctcgt ccatgccgcc
      721 ggtggagtgg cggccctcgg cgcgttcgta ctgttccacg atggtgtagt cctcgttgtg
      781 ggaggtgatg tccaacttga tgttgacgtt gtaggcgccg ggcagctgca cgggcttctt
      841 ggccttgtag gtggtcttga cctcagcgtc gtagtggccg ccgtccttca gcttcagcct
      901 ctgcttgatc tcgcccttca gggcgccgtc ctcggggtac atccgctcgg aggaggcctc
      961 ccagcccatg gtcttcttct gcattacggg gccgtcggag gggaagttgg tgccgcgcag
     1021 cttcaccttg tagatgaact cgccgtcc
//
