LOCUS       sequence-102147-         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GeneGuard genomic cassette: for constructing Delta-thyA::RBS B repA,
            Epsilon, FRT-kanR-FRT (low-copy support for ColE2 COR). Will support
            pSEVAT7Z..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Wright O, Delmans M, Stan GB, Ellis T
  TITLE     GeneGuard: a Modular Plasmid System Designed for Biosafety.
  JOURNAL   ACS Synth Biol. 2014 May 13.
  PUBMED    24847673
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2014 May 13."
COMMENT     Sequence Label: pSEVA12TEC-B
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(387..420)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     RBS             715..723
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     RBS             764..772
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 atccatcttg ttcaatcatg cgaaacgatc ctcatcctgt ctcttgatca gatcttgatc
       61 ccctgcgcca tcagatcctt ggcggcaaga aagccatcca gtttactttg cagggcttcc
      121 caaccttacc agagggcgcc ccagctggca attccggttc gcttgctgtc cataaaaccg
      181 cccagtctag ctatcgccat gtaagcccac tgcaagctac ctgctttctc tttgcgcttg
      241 cgttttccct tgtccagata gcccagtagc tgacattcat ccggggtcag caccgtttct
      301 gcggactggc tttctacgtg ttccgcttcc tttagcagcc cttgcgccct gagtgcttgc
      361 ggcagcgtga gcttcaaaag cgctctgaag ttcctatact ttctagagaa taggaacttc
      421 gaactgcagg tcgactcaag ccactttctc tttattcaaa acttgttttg agtaacgatt
      481 cattgaccgc caatactcat gaacggcttg taattcttct aaagaataat caaaaagatt
      541 tacacgtttt gcaagcttta attggtttaa taaattgact tccaataatt gagagtcatt
      601 tttatttaat tcatggttca aaacatagtt taatactcga ttataaacgc ttgccgataa
      661 ttcgttaatg atctctattt caaatgtttt ttcatacgta actgccatta gaaaacctcc
      721 ttagcatgat taagatgttt cttcaacatc tcgcatggtt gttacctcct tacctctggt
      781 ctccacacat tatacctata ggttagactt taagtcaata ctctttttcc cgggtataaa
      841 cgcagaaagg cccacccgaa
//
